Dynamics of human replication protein A subunit distribution and partitioning in the cell cycle

Kuruganti G. Murti, Da Cheng He, Bill R. Brinkley, Rebecca Scott, Suk-Hee Lee

Research output: Contribution to journalArticle

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Abstract

Human replication protein A (RPA) is a three-subunit protein complex involved in DNA replication, repair, and recombination. To gain insight into the dynamics of subunit assembly, we examined the subcellular distribution of RPA subunits (p70, p34, and p11) during the cell cycle. All three subunits colocalized in G1 and S phases, showing a diffuse nuclear distribution in G1 but a dot-like nuclear pattern in S phase. During S phase, the subunits showed a pattern reminiscent of the replication granules/factories described by others as sites of replication machinery. In metaphase, p70 preferentially associated with the spindle poles, p34 was found on chromosomes, and p11 remained in the cytoplasm. In telophase, p70 and p34 appeared in the forming daughter nuclei; p11 remained in the cytoplasm until G1. Among the three subunits only p34 was associated with the nuclear matrix and this association persisted throughout the cell cycle. We conclude that (i) RPA complex assembly is differentially regulated, (ii) the replication machinery may be anchored to the nuclear matrix, and (iii) RPA subunits partition during mitosis and sort into daughter nuclei by different routes.

Original languageEnglish (US)
Pages (from-to)279-289
Number of pages11
JournalExperimental Cell Research
Volume223
Issue number2
DOIs
StatePublished - Mar 15 1996
Externally publishedYes

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Replication Protein A
Protein Subunits
S Phase
Nuclear Matrix
Cell Cycle
Cytoplasm
Spindle Poles
Telophase
G1 Phase
Metaphase
DNA Replication
Mitosis
DNA Repair
Genetic Recombination
Chromosomes
human RPA1 protein

ASJC Scopus subject areas

  • Cell Biology

Cite this

Dynamics of human replication protein A subunit distribution and partitioning in the cell cycle. / Murti, Kuruganti G.; He, Da Cheng; Brinkley, Bill R.; Scott, Rebecca; Lee, Suk-Hee.

In: Experimental Cell Research, Vol. 223, No. 2, 15.03.1996, p. 279-289.

Research output: Contribution to journalArticle

Murti, Kuruganti G. ; He, Da Cheng ; Brinkley, Bill R. ; Scott, Rebecca ; Lee, Suk-Hee. / Dynamics of human replication protein A subunit distribution and partitioning in the cell cycle. In: Experimental Cell Research. 1996 ; Vol. 223, No. 2. pp. 279-289.
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