Dysregulated bcl-2 expression inhibits apoptosis but not differentiation of retinoic acid-induced HL-60 granulocytes

Ulie R. Park, Kent Robertson, Dennis D. Hickstein, Schickwann Tsai, David M. Hockenbery, Steven J. Collins

Research output: Contribution to journalArticle

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Abstract

The bcl-2 proto-oncogene appears to contribute to the development of certain malignancies by inhibiting programmed cell death (apoptosis). Mature granulocytes show a markedly limited life span and rapidly undergo apoptosis. To further define the relationship between apoptosis and granulocyte differentiation, we used retroviral vector-mediated gene transduction to introduce the normal bcl-2 gene into the HL-60 myeloid leukemia cell line and determined the response of these bcl-2-transduced HL-60 cells to the induction of granulocyte differentiation by retinoic acid (RA). Although the bcl-2-transduced HL-60 cells showed the same differentiative response to RA as did the parental HL-60 cells, the life span of the RA-induced, bcl-2- transduced HL-60 granulocytes was markedly prolonged compared with that of the RA-induced parental HL-60 granulocytes. DNA fragmentation studies indicate that this prolonged life span resulted from diminished apoptosis in the bcl-2-transduced cells. These studies indicate that bcl-2 is involved in regulating apoptosis in maturing granulocytes. Because bcl-2 overexpression did not interfere with RA-induced granulocyte differentiation, it appears that granulocyte differentiation and apoptosis are under distinct and separate regulatory controls.

Original languageEnglish (US)
Pages (from-to)440-445
Number of pages6
JournalBlood
Volume84
Issue number2
StatePublished - Jul 15 1994
Externally publishedYes

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Tretinoin
Granulocytes
Apoptosis
HL-60 Cells
Genes
Cell death
bcl-2 Genes
Myeloid Leukemia
Proto-Oncogenes
DNA Fragmentation
Myeloid Cells
Cells
Cell Death
DNA
Cell Line
Neoplasms

ASJC Scopus subject areas

  • Hematology

Cite this

Park, U. R., Robertson, K., Hickstein, D. D., Tsai, S., Hockenbery, D. M., & Collins, S. J. (1994). Dysregulated bcl-2 expression inhibits apoptosis but not differentiation of retinoic acid-induced HL-60 granulocytes. Blood, 84(2), 440-445.

Dysregulated bcl-2 expression inhibits apoptosis but not differentiation of retinoic acid-induced HL-60 granulocytes. / Park, Ulie R.; Robertson, Kent; Hickstein, Dennis D.; Tsai, Schickwann; Hockenbery, David M.; Collins, Steven J.

In: Blood, Vol. 84, No. 2, 15.07.1994, p. 440-445.

Research output: Contribution to journalArticle

Park, UR, Robertson, K, Hickstein, DD, Tsai, S, Hockenbery, DM & Collins, SJ 1994, 'Dysregulated bcl-2 expression inhibits apoptosis but not differentiation of retinoic acid-induced HL-60 granulocytes', Blood, vol. 84, no. 2, pp. 440-445.
Park, Ulie R. ; Robertson, Kent ; Hickstein, Dennis D. ; Tsai, Schickwann ; Hockenbery, David M. ; Collins, Steven J. / Dysregulated bcl-2 expression inhibits apoptosis but not differentiation of retinoic acid-induced HL-60 granulocytes. In: Blood. 1994 ; Vol. 84, No. 2. pp. 440-445.
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