E-cadherin dis-engagement activates the Rap1 GTPase

Sirisha Asuri, Jingliang Yan, Nivanka C. Paranavitana, Lawrence Quilliam

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

E-cadherin based adherens junctions are finely regulated by multiple cellular signaling events. Here we show that the Ras-related Rap1 GTPase is enriched in regions of nascent cell-cell contacts and strengthens E-cadherin junctions: constitutively active Rap1 expressing MDCK cells exhibit increased junctional contact and resisted calcium depletion-induced cell-cell junction disruption. E-cadherin disengagement activated Rap1 and this correlated with E-cadherin association with the Rap GEFs, C3G and PDZ-GEF I. PDZ-GEF I associated with E-cadherin and β-catenin whereas C3G interaction with E-cadherin did not involve β-catenin. Knockdown of PDZ-GEF I in MDCK cells decreased Rap1 activity following E-cadherin junction disruption. We hereby show that Rap1 plays a role in the maintenance and repair of E-cadherin junctions and is activated via an "outside-in" signaling pathway initiated by E-cadherin and mediated at least in part by PDZ-GEF I.

Original languageEnglish
Pages (from-to)1027-1037
Number of pages11
JournalJournal of Cellular Biochemistry
Volume105
Issue number4
DOIs
StatePublished - Jan 11 2008

Fingerprint

GTP Phosphohydrolases
Cadherins
Catenins
Madin Darby Canine Kidney Cells
Cell signaling
Adherens Junctions
Intercellular Junctions
Monomeric GTP-Binding Proteins
Repair
Maintenance
Calcium

Keywords

  • Adherens junction
  • C3G
  • E-cadherin
  • Guanine nucleotide exchange factor
  • PDZ-GEF
  • Rap1

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

E-cadherin dis-engagement activates the Rap1 GTPase. / Asuri, Sirisha; Yan, Jingliang; Paranavitana, Nivanka C.; Quilliam, Lawrence.

In: Journal of Cellular Biochemistry, Vol. 105, No. 4, 11.01.2008, p. 1027-1037.

Research output: Contribution to journalArticle

Asuri, Sirisha ; Yan, Jingliang ; Paranavitana, Nivanka C. ; Quilliam, Lawrence. / E-cadherin dis-engagement activates the Rap1 GTPase. In: Journal of Cellular Biochemistry. 2008 ; Vol. 105, No. 4. pp. 1027-1037.
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