Early assessment of burn severity in human tissue ex vivo with multi-wavelength spatial frequency domain imaging

Chien Poon, Ulas Sunar, Daniel J. Rohrbach, Smita Krishnamurthy, Thomas Olsen, Michael Kent, Nathan M. Weir, Richard Simman, Jeffrey Travers

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Early knowledge about burn severity and depth can lead to improved outcome for patients. In this study, we investigated the change in optical properties in ex vivo human skin following thermal burn injuries. Human skin removed during body contouring procedures was subjected to thermal burn injury for either 10 or 60 s. Multi-wavelength spatial frequency domain imaging (SFDI) measurements were performed on each sample and the optical properties (absorption and scattering parameters) were obtained at each wavelength. Multi-wavelength fitting was used to quantify absorption and scattering parameters, and these parameters were compared to histologic assessments of burn depth related to burn severity. Our results indicated substantial changes in optical scattering parameters and these changes correlated well with the burn severity and depth, and fit closely with previously reported studies using porcine in vivo models. This study provides the characterization of thermal burn injury on human skin ex vivo by using the optical method of SFDI with high sensitivity and specificity. This preclinical human model system without live animals could have uses in testing the imaging parameters of other skin injuries, including from caustic agents.

Original languageEnglish (US)
Pages (from-to)251-254
Number of pages4
JournalToxicology in Vitro
Volume52
DOIs
StatePublished - Oct 1 2018
Externally publishedYes

Fingerprint

Skin
Scattering parameters
Tissue
Imaging techniques
Wavelength
Hot Temperature
Wounds and Injuries
Optical properties
Caustics
Animals
Swine
Sensitivity and Specificity
Testing

Keywords

  • Animal testing alternatives
  • Human skin
  • Mesoscopic imaging
  • Thermal burn

ASJC Scopus subject areas

  • Toxicology

Cite this

Early assessment of burn severity in human tissue ex vivo with multi-wavelength spatial frequency domain imaging. / Poon, Chien; Sunar, Ulas; Rohrbach, Daniel J.; Krishnamurthy, Smita; Olsen, Thomas; Kent, Michael; Weir, Nathan M.; Simman, Richard; Travers, Jeffrey.

In: Toxicology in Vitro, Vol. 52, 01.10.2018, p. 251-254.

Research output: Contribution to journalArticle

Poon, C, Sunar, U, Rohrbach, DJ, Krishnamurthy, S, Olsen, T, Kent, M, Weir, NM, Simman, R & Travers, J 2018, 'Early assessment of burn severity in human tissue ex vivo with multi-wavelength spatial frequency domain imaging', Toxicology in Vitro, vol. 52, pp. 251-254. https://doi.org/10.1016/j.tiv.2018.05.015
Poon, Chien ; Sunar, Ulas ; Rohrbach, Daniel J. ; Krishnamurthy, Smita ; Olsen, Thomas ; Kent, Michael ; Weir, Nathan M. ; Simman, Richard ; Travers, Jeffrey. / Early assessment of burn severity in human tissue ex vivo with multi-wavelength spatial frequency domain imaging. In: Toxicology in Vitro. 2018 ; Vol. 52. pp. 251-254.
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AU - Olsen, Thomas

AU - Kent, Michael

AU - Weir, Nathan M.

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AU - Travers, Jeffrey

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