Early dynamic fate changes in haemogenic endothelium characterized at the single-cell level

Gemma Swiers, Claudia Baumann, John O'Rourke, Eleni Giannoulatou, Stephen Taylor, Anagha Joshi, Victoria Moignard, Cristina Pina, Thomas Bee, Konstantinos D. Kokkaliaris, Momoko Yoshimoto, Mervin Yoder, Jon Frampton, Timm Schroeder, Tariq Enver, Berthold Göttgens, Marella F T R De Bruijn

Research output: Contribution to journalArticle

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Abstract

Haematopoietic stem cells (HSCs) are the founding cells of the adult haematopoietic system, born during ontogeny from a specialized subset of endothelium, the haemogenic endothelium (HE) via an endothelial-to- haematopoietic transition (EHT). Although recently imaged in real time, the underlying mechanism of EHT is still poorly understood. We have generated a Runx1 +23 enhancer-reporter transgenic mouse (23GFP) for the prospective isolation of HE throughout embryonic development. Here we perform functional analysis of over 1,800 and transcriptional analysis of 268 single 23GFP + HE cells to explore the onset of EHT at the single-cell level. We show that initiation of the haematopoietic programme occurs in cells still embedded in the endothelial layer, and is accompanied by a previously unrecognized early loss of endothelial potential before HSCs emerge. Our data therefore provide important insights on the timeline of early haematopoietic commitment.

Original languageEnglish (US)
Article number2924
JournalNature Communications
Volume4
DOIs
StatePublished - Dec 11 2013

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endothelium
Stem cells
Endothelium
Functional analysis
stem cells
Hematopoietic Stem Cells
cells
hematopoietic system
ontogeny
functional analysis
Hematopoietic System
Transgenic Mice
set theory
Embryonic Development
mice
isolation
TimeLine

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Chemistry(all)
  • Physics and Astronomy(all)

Cite this

Swiers, G., Baumann, C., O'Rourke, J., Giannoulatou, E., Taylor, S., Joshi, A., ... De Bruijn, M. F. T. R. (2013). Early dynamic fate changes in haemogenic endothelium characterized at the single-cell level. Nature Communications, 4, [2924]. https://doi.org/10.1038/ncomms3924

Early dynamic fate changes in haemogenic endothelium characterized at the single-cell level. / Swiers, Gemma; Baumann, Claudia; O'Rourke, John; Giannoulatou, Eleni; Taylor, Stephen; Joshi, Anagha; Moignard, Victoria; Pina, Cristina; Bee, Thomas; Kokkaliaris, Konstantinos D.; Yoshimoto, Momoko; Yoder, Mervin; Frampton, Jon; Schroeder, Timm; Enver, Tariq; Göttgens, Berthold; De Bruijn, Marella F T R.

In: Nature Communications, Vol. 4, 2924, 11.12.2013.

Research output: Contribution to journalArticle

Swiers, G, Baumann, C, O'Rourke, J, Giannoulatou, E, Taylor, S, Joshi, A, Moignard, V, Pina, C, Bee, T, Kokkaliaris, KD, Yoshimoto, M, Yoder, M, Frampton, J, Schroeder, T, Enver, T, Göttgens, B & De Bruijn, MFTR 2013, 'Early dynamic fate changes in haemogenic endothelium characterized at the single-cell level', Nature Communications, vol. 4, 2924. https://doi.org/10.1038/ncomms3924
Swiers G, Baumann C, O'Rourke J, Giannoulatou E, Taylor S, Joshi A et al. Early dynamic fate changes in haemogenic endothelium characterized at the single-cell level. Nature Communications. 2013 Dec 11;4. 2924. https://doi.org/10.1038/ncomms3924
Swiers, Gemma ; Baumann, Claudia ; O'Rourke, John ; Giannoulatou, Eleni ; Taylor, Stephen ; Joshi, Anagha ; Moignard, Victoria ; Pina, Cristina ; Bee, Thomas ; Kokkaliaris, Konstantinos D. ; Yoshimoto, Momoko ; Yoder, Mervin ; Frampton, Jon ; Schroeder, Timm ; Enver, Tariq ; Göttgens, Berthold ; De Bruijn, Marella F T R. / Early dynamic fate changes in haemogenic endothelium characterized at the single-cell level. In: Nature Communications. 2013 ; Vol. 4.
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