Ectopic overexpression of the homeobox gene xgbx-2 during xenopus development

Anton W. Neff, Michael King

Research output: Contribution to journalArticle

Abstract

The newly described homeobox gene Xgbx-2 is expressed during gastrulation and neurulation in the dorsolateral ectoderm. Gbx-2 becomes restricted to the neuroectoderm at the mid/hindbrain boundary and in the epidermis ventral and lateral to the neural plate. To assess function during early development we injected synthetic Xgbx-2 RNA into specific blastomeres at the 4 and 8 cell stage. Injection of Xgbx-2 (0.1 to 0.8 ng) into both dorsal blastomeres at the 4 cell stage results in a high incidence of gastrulation defects (open backs) and posthatchlings with reduced dorsal and anterior structures (eye size and cement gland size were reduced). The Xgbx-2 phenotype is dependent on the blastomeres that are injected. The two ventral blastomeres at the 4 cell stage and the two vegetal/ventral blastomeres at the 8 cell stage are the least affected. Surprisingly, the two dorsal blastomeres at the 4 cell stage are more sensitive to Xgbx-2 than either the two animal/dorsal or vegetal/dorsal blastomeres at the 8 cell stage. These results are consistent with the working hypothesis that the function of Xgbx-2 is inhibitory, affecting gastrulation and anterior/dorsal development, leading to the establishment of the mid/hindbrain boundary.

Original languageEnglish
JournalFASEB Journal
Volume10
Issue number3
StatePublished - 1996
Externally publishedYes

Fingerprint

Blastomeres
blastomeres
homeotic genes
Homeobox Genes
Xenopus
Genes
Gastrulation
Cements
Animals
Neural Plate
Rhombencephalon
RNA
Defects
cells
Neurulation
brain
Ectoderm
epidermis (animal)
back (body region)
Epidermis

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Ectopic overexpression of the homeobox gene xgbx-2 during xenopus development. / Neff, Anton W.; King, Michael.

In: FASEB Journal, Vol. 10, No. 3, 1996.

Research output: Contribution to journalArticle

@article{def34ccfed3e42fd99105994b9a3792d,
title = "Ectopic overexpression of the homeobox gene xgbx-2 during xenopus development",
abstract = "The newly described homeobox gene Xgbx-2 is expressed during gastrulation and neurulation in the dorsolateral ectoderm. Gbx-2 becomes restricted to the neuroectoderm at the mid/hindbrain boundary and in the epidermis ventral and lateral to the neural plate. To assess function during early development we injected synthetic Xgbx-2 RNA into specific blastomeres at the 4 and 8 cell stage. Injection of Xgbx-2 (0.1 to 0.8 ng) into both dorsal blastomeres at the 4 cell stage results in a high incidence of gastrulation defects (open backs) and posthatchlings with reduced dorsal and anterior structures (eye size and cement gland size were reduced). The Xgbx-2 phenotype is dependent on the blastomeres that are injected. The two ventral blastomeres at the 4 cell stage and the two vegetal/ventral blastomeres at the 8 cell stage are the least affected. Surprisingly, the two dorsal blastomeres at the 4 cell stage are more sensitive to Xgbx-2 than either the two animal/dorsal or vegetal/dorsal blastomeres at the 8 cell stage. These results are consistent with the working hypothesis that the function of Xgbx-2 is inhibitory, affecting gastrulation and anterior/dorsal development, leading to the establishment of the mid/hindbrain boundary.",
author = "Neff, {Anton W.} and Michael King",
year = "1996",
language = "English",
volume = "10",
journal = "FASEB Journal",
issn = "0892-6638",
publisher = "FASEB",
number = "3",

}

TY - JOUR

T1 - Ectopic overexpression of the homeobox gene xgbx-2 during xenopus development

AU - Neff, Anton W.

AU - King, Michael

PY - 1996

Y1 - 1996

N2 - The newly described homeobox gene Xgbx-2 is expressed during gastrulation and neurulation in the dorsolateral ectoderm. Gbx-2 becomes restricted to the neuroectoderm at the mid/hindbrain boundary and in the epidermis ventral and lateral to the neural plate. To assess function during early development we injected synthetic Xgbx-2 RNA into specific blastomeres at the 4 and 8 cell stage. Injection of Xgbx-2 (0.1 to 0.8 ng) into both dorsal blastomeres at the 4 cell stage results in a high incidence of gastrulation defects (open backs) and posthatchlings with reduced dorsal and anterior structures (eye size and cement gland size were reduced). The Xgbx-2 phenotype is dependent on the blastomeres that are injected. The two ventral blastomeres at the 4 cell stage and the two vegetal/ventral blastomeres at the 8 cell stage are the least affected. Surprisingly, the two dorsal blastomeres at the 4 cell stage are more sensitive to Xgbx-2 than either the two animal/dorsal or vegetal/dorsal blastomeres at the 8 cell stage. These results are consistent with the working hypothesis that the function of Xgbx-2 is inhibitory, affecting gastrulation and anterior/dorsal development, leading to the establishment of the mid/hindbrain boundary.

AB - The newly described homeobox gene Xgbx-2 is expressed during gastrulation and neurulation in the dorsolateral ectoderm. Gbx-2 becomes restricted to the neuroectoderm at the mid/hindbrain boundary and in the epidermis ventral and lateral to the neural plate. To assess function during early development we injected synthetic Xgbx-2 RNA into specific blastomeres at the 4 and 8 cell stage. Injection of Xgbx-2 (0.1 to 0.8 ng) into both dorsal blastomeres at the 4 cell stage results in a high incidence of gastrulation defects (open backs) and posthatchlings with reduced dorsal and anterior structures (eye size and cement gland size were reduced). The Xgbx-2 phenotype is dependent on the blastomeres that are injected. The two ventral blastomeres at the 4 cell stage and the two vegetal/ventral blastomeres at the 8 cell stage are the least affected. Surprisingly, the two dorsal blastomeres at the 4 cell stage are more sensitive to Xgbx-2 than either the two animal/dorsal or vegetal/dorsal blastomeres at the 8 cell stage. These results are consistent with the working hypothesis that the function of Xgbx-2 is inhibitory, affecting gastrulation and anterior/dorsal development, leading to the establishment of the mid/hindbrain boundary.

UR - http://www.scopus.com/inward/record.url?scp=33749275828&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33749275828&partnerID=8YFLogxK

M3 - Article

VL - 10

JO - FASEB Journal

JF - FASEB Journal

SN - 0892-6638

IS - 3

ER -