Effect of 1,25-dihydroxyvitamin D3 on alkaline phosphatase activity and collagen synthesis in osteoblastic cells, clone MC3T3-El1

Noriyoshi Kurihara, Katsumi Ikeda, Yoshiyuki Hakeda, Mauri Tsunoi, Norihiko Maeda, Masayoshi Kumegawa

Research output: Contribution to journalArticle

61 Citations (Scopus)

Abstract

A stimulative effect of 1,25-dihydroxyvitamin D3 was tested on osteoblastic cells, clone MC3T3-El, cultured in serum-free medium with 0.1% bovine serum albumin. This steroid increased alkaline phosphatase activity in a dose-related fashion. The steroid also stimulated dose-dependently collagen and non-collagen protein syntheses, their maximal effects being observed at 12 and 24 h, respectively. The incorporation of [3H]-proline into collagen or non-collagen protein in cells exposed to this steroid for 12 h was 2.9 or 1.9-fold over that of control cultures, respectively. These results strongly indicate the stimulative effects of 1,25-dihydroxyvitamin D3 on the differentiation of osteoblasts in vitro.

Original languageEnglish (US)
Pages (from-to)767-771
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume119
Issue number2
DOIs
StatePublished - Mar 15 1984
Externally publishedYes

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Clone cells
Calcitriol
Alkaline Phosphatase
Collagen
Clone Cells
Steroids
Osteoblasts
Serum-Free Culture Media
Bovine Serum Albumin
Proline
Proteins

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Effect of 1,25-dihydroxyvitamin D3 on alkaline phosphatase activity and collagen synthesis in osteoblastic cells, clone MC3T3-El1. / Kurihara, Noriyoshi; Ikeda, Katsumi; Hakeda, Yoshiyuki; Tsunoi, Mauri; Maeda, Norihiko; Kumegawa, Masayoshi.

In: Biochemical and Biophysical Research Communications, Vol. 119, No. 2, 15.03.1984, p. 767-771.

Research output: Contribution to journalArticle

Kurihara, Noriyoshi ; Ikeda, Katsumi ; Hakeda, Yoshiyuki ; Tsunoi, Mauri ; Maeda, Norihiko ; Kumegawa, Masayoshi. / Effect of 1,25-dihydroxyvitamin D3 on alkaline phosphatase activity and collagen synthesis in osteoblastic cells, clone MC3T3-El1. In: Biochemical and Biophysical Research Communications. 1984 ; Vol. 119, No. 2. pp. 767-771.
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