Effect of alloxan-induced diabetes on the glomerular filtration barrier of the rat

Andrew Evan, F. C. Luft

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

To elucidate morphologic changes in glomeruli of rats made diabetic with alloxan 40 mg/kg, we examined the filtration barrier with light microscopy, transmission (TEM), and scanning electron microscopy (SEM). The rats were killed at 10 or 20 weeks. The presence of diabetes was verified by blood and glucose measurements. In vivo pefusion was carried out through the left ventricle with gluteraldehyde in cacodylate buffer. SEM and TEM were performed by conventional techniques. SEM revealed striking changes in both the epithelial and endothelial surfaces which were focal and progressive with time. The epithelial surfaces showed podocyte fusion with areas of frank necrosis. Occasional areas of regeneration were seen. The endothelium showed a loss in number and decrease in size of fenestrate, proliferation of microvilli, and appearance of irregular cellular processes. TEM confirmed the presence of cellular changes. The basement membrane was irregularly thickened and the mesangium ws more prominent than in control animals. We conclude that not only is the basement membrane affected by diabetes, but also important endothelial and epithelial cell changes occur as well. These changes are best appreciated by SEM and have not been previously described.

Original languageEnglish
Pages (from-to)257-264
Number of pages8
JournalRenal Physiology
Volume3
Issue number1-6
StatePublished - 1980

Fingerprint

Glomerular Filtration Barrier
Experimental Diabetes Mellitus
Electron Scanning Microscopy
Basement Membrane
Cacodylic Acid
Podocytes
Scanning Transmission Electron Microscopy
Alloxan
Microvilli
Heart Ventricles
Endothelium
Blood Glucose
Regeneration
Microscopy
Buffers
Necrosis
Endothelial Cells
Epithelial Cells
Light

ASJC Scopus subject areas

  • Physiology
  • Nephrology

Cite this

Effect of alloxan-induced diabetes on the glomerular filtration barrier of the rat. / Evan, Andrew; Luft, F. C.

In: Renal Physiology, Vol. 3, No. 1-6, 1980, p. 257-264.

Research output: Contribution to journalArticle

@article{b41dade98f444f51b30a8a01f05ef25a,
title = "Effect of alloxan-induced diabetes on the glomerular filtration barrier of the rat",
abstract = "To elucidate morphologic changes in glomeruli of rats made diabetic with alloxan 40 mg/kg, we examined the filtration barrier with light microscopy, transmission (TEM), and scanning electron microscopy (SEM). The rats were killed at 10 or 20 weeks. The presence of diabetes was verified by blood and glucose measurements. In vivo pefusion was carried out through the left ventricle with gluteraldehyde in cacodylate buffer. SEM and TEM were performed by conventional techniques. SEM revealed striking changes in both the epithelial and endothelial surfaces which were focal and progressive with time. The epithelial surfaces showed podocyte fusion with areas of frank necrosis. Occasional areas of regeneration were seen. The endothelium showed a loss in number and decrease in size of fenestrate, proliferation of microvilli, and appearance of irregular cellular processes. TEM confirmed the presence of cellular changes. The basement membrane was irregularly thickened and the mesangium ws more prominent than in control animals. We conclude that not only is the basement membrane affected by diabetes, but also important endothelial and epithelial cell changes occur as well. These changes are best appreciated by SEM and have not been previously described.",
author = "Andrew Evan and Luft, {F. C.}",
year = "1980",
language = "English",
volume = "3",
pages = "257--264",
journal = "Kidney and Blood Pressure Research",
issn = "1420-4096",
publisher = "S. Karger AG",
number = "1-6",

}

TY - JOUR

T1 - Effect of alloxan-induced diabetes on the glomerular filtration barrier of the rat

AU - Evan, Andrew

AU - Luft, F. C.

PY - 1980

Y1 - 1980

N2 - To elucidate morphologic changes in glomeruli of rats made diabetic with alloxan 40 mg/kg, we examined the filtration barrier with light microscopy, transmission (TEM), and scanning electron microscopy (SEM). The rats were killed at 10 or 20 weeks. The presence of diabetes was verified by blood and glucose measurements. In vivo pefusion was carried out through the left ventricle with gluteraldehyde in cacodylate buffer. SEM and TEM were performed by conventional techniques. SEM revealed striking changes in both the epithelial and endothelial surfaces which were focal and progressive with time. The epithelial surfaces showed podocyte fusion with areas of frank necrosis. Occasional areas of regeneration were seen. The endothelium showed a loss in number and decrease in size of fenestrate, proliferation of microvilli, and appearance of irregular cellular processes. TEM confirmed the presence of cellular changes. The basement membrane was irregularly thickened and the mesangium ws more prominent than in control animals. We conclude that not only is the basement membrane affected by diabetes, but also important endothelial and epithelial cell changes occur as well. These changes are best appreciated by SEM and have not been previously described.

AB - To elucidate morphologic changes in glomeruli of rats made diabetic with alloxan 40 mg/kg, we examined the filtration barrier with light microscopy, transmission (TEM), and scanning electron microscopy (SEM). The rats were killed at 10 or 20 weeks. The presence of diabetes was verified by blood and glucose measurements. In vivo pefusion was carried out through the left ventricle with gluteraldehyde in cacodylate buffer. SEM and TEM were performed by conventional techniques. SEM revealed striking changes in both the epithelial and endothelial surfaces which were focal and progressive with time. The epithelial surfaces showed podocyte fusion with areas of frank necrosis. Occasional areas of regeneration were seen. The endothelium showed a loss in number and decrease in size of fenestrate, proliferation of microvilli, and appearance of irregular cellular processes. TEM confirmed the presence of cellular changes. The basement membrane was irregularly thickened and the mesangium ws more prominent than in control animals. We conclude that not only is the basement membrane affected by diabetes, but also important endothelial and epithelial cell changes occur as well. These changes are best appreciated by SEM and have not been previously described.

UR - http://www.scopus.com/inward/record.url?scp=0019274629&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0019274629&partnerID=8YFLogxK

M3 - Article

VL - 3

SP - 257

EP - 264

JO - Kidney and Blood Pressure Research

JF - Kidney and Blood Pressure Research

SN - 1420-4096

IS - 1-6

ER -