Effect of bioactive borate glass microstructure on bone regeneration, angiogenesis, and hydroxyapatite conversion in a rat calvarial defect model

Lianxiang Bi, Mohamed N. Rahaman, Delbert E. Day, Zackary Brown, Christopher Samujh, Xin Liu, Ali Mohammadkhah, Vladimir Dusevich, J. David Eick, Lynda Bonewald

Research output: Contribution to journalArticle

66 Citations (Scopus)

Abstract

Borate bioactive glasses are biocompatible and enhance new bone formation, but the effect of their microstructure on bone regeneration has received little attention. In this study scaffolds of borate bioactive glass (1393B3) with three different microstructures (trabecular, fibrous, and oriented) were compared for their capacity to regenerate bone in a rat calvarial defect model. 12 weeks post-implantation the amount of new bone, mineralization, and blood vessel area in the scaffolds were evaluated using histomorphometric analysis and scanning electron microscopy. The amount of new bone formed was 33%, 23%, and 15%, respectively, of the total defect area for the trabecular, oriented, and fibrous microstructures. In comparison, the percent new bone formed in implants composed of silicate 45S5 bioactive glass particles (250-300 lm) was 19%. Doping the borate glass with copper (0.4 wt.% CuO) had little effect on bone regeneration in the trabecular and oriented scaffolds, but significantly enhanced bone regeneration in the fibrous scaffolds (from 15 to 33%). The scaffolds were completely converted to hydroxyapatite within the 12 week implantation. The amount of hydroxyapatite formed, 22%, 35%, and 48%, respectively, for the trabecular, oriented, and fibrous scaffolds, increased with increasing volume fraction of glass in the as-fabricated scaffold. Blood vessels infiltrated into all the scaffolds, but the trabecular scaffolds had a higher average blood vessel area compared with the oriented and fibrous scaffolds. While all three scaffold microstructures were effective in supporting bone regeneration, the trabecular scaffolds supported more bone formation and may be more promising in bone repair.

Original languageEnglish (US)
Pages (from-to)8015-8026
Number of pages12
JournalActa Biomaterialia
Volume9
Issue number8
DOIs
StatePublished - Aug 2013
Externally publishedYes

Fingerprint

Borates
Bone Regeneration
Durapatite
Hydroxyapatite
Scaffolds
Glass
Rats
Bone
Blood Vessels
Bone and Bones
Defects
Microstructure
Osteogenesis
Physiologic Calcification
Silicates
Bioactive glass
Blood vessels
Electron Scanning Microscopy
Copper
Volume fraction

Keywords

  • Bioactive glass
  • Bone regeneration
  • Borate glass
  • Scaffold

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering
  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Medicine(all)

Cite this

Effect of bioactive borate glass microstructure on bone regeneration, angiogenesis, and hydroxyapatite conversion in a rat calvarial defect model. / Bi, Lianxiang; Rahaman, Mohamed N.; Day, Delbert E.; Brown, Zackary; Samujh, Christopher; Liu, Xin; Mohammadkhah, Ali; Dusevich, Vladimir; Eick, J. David; Bonewald, Lynda.

In: Acta Biomaterialia, Vol. 9, No. 8, 08.2013, p. 8015-8026.

Research output: Contribution to journalArticle

Bi, Lianxiang ; Rahaman, Mohamed N. ; Day, Delbert E. ; Brown, Zackary ; Samujh, Christopher ; Liu, Xin ; Mohammadkhah, Ali ; Dusevich, Vladimir ; Eick, J. David ; Bonewald, Lynda. / Effect of bioactive borate glass microstructure on bone regeneration, angiogenesis, and hydroxyapatite conversion in a rat calvarial defect model. In: Acta Biomaterialia. 2013 ; Vol. 9, No. 8. pp. 8015-8026.
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AU - Brown, Zackary

AU - Samujh, Christopher

AU - Liu, Xin

AU - Mohammadkhah, Ali

AU - Dusevich, Vladimir

AU - Eick, J. David

AU - Bonewald, Lynda

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