Effect of Bordetella endotoxin upon mitochondrial respiration and energized processes

Robert Harris, D. L. Harris, D. E. Green

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Endotoxin isolated from Bordetella bronchiseptica 22067 by the Boivin method markedly affected respiration, certain energized processes, and the morphology of beef-heart mitochondria. Interaction of endotoxin with intact mitochondria resulted in loss of respiratory control, inhibition of the coupling of phosphorylation to terminal electron transfer, inhibition of respiration in presence either of DPNH-linked substrates or of succinate, stimulation of respiration with ascorbate-TPD4 4 Abbreviation used: HBHM, heavy beef-heart mitochondria; ETPH, phosphorylating submitochondrial electron transfer particles; Pi, inorganic phosphate; EDTA, ethylenediaminetetracetate; TPD, N,N,N′,N′,-tetramethyl-p-phenylenediamine; m-Cl-CCP, carbonyl cyanide m-chloropenylhydrozone; DNP, 2,4-dinitrophenol. as electron donor, inhibition of Ca2+ translocation whether energized by electron transfer or by ATP, but stimulation of Mg2+ translocation energized by ascorbate plus TPD. Changes in the morphology of the inner membrane as a result of interaction of the mitochondrial membrane system with endotoxin were readily demonstrable. A submitochondrial preparation composed predominantly of inner membrane (ETPH) was much more resistant than intact mitochondria to the action of endotoxin; higher concentrations of endotoxin were necessary for inhibition of respiration and energized processes. The action of B. bronchiseptica 22067 endotoxin mimicked, in part, that of other reagents, such as the murine toxin of Pasteurella pestis, Zn2+, certain basic proteins, and parathyroid hormone.

Original languageEnglish (US)
Pages (from-to)219-230
Number of pages12
JournalArchives of Biochemistry and Biophysics
Volume128
Issue number1
StatePublished - Oct 1968
Externally publishedYes

Fingerprint

Bordetella
Endotoxins
Respiration
Mitochondria
Bordetella bronchiseptica
Electrons
Heart Mitochondria
Beef
Temperature programmed desorption
Membranes
Yersinia pestis
2,4-Dinitrophenol
Phosphorylation
Mitochondrial Membranes
Succinic Acid
Parathyroid Hormone
Edetic Acid
Adenosine Triphosphate
Phosphates
Substrates

ASJC Scopus subject areas

  • Molecular Biology
  • Biophysics
  • Biochemistry

Cite this

Effect of Bordetella endotoxin upon mitochondrial respiration and energized processes. / Harris, Robert; Harris, D. L.; Green, D. E.

In: Archives of Biochemistry and Biophysics, Vol. 128, No. 1, 10.1968, p. 219-230.

Research output: Contribution to journalArticle

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