Effect of carbon nanoparticles on renal epithelial cell structure, barrier function, and protein expression

Bonnie L. Blazer-Yost, Amiraj Banga, Adam Amos, Ellen Chernoff, Xianyin Lai, Cheng Li, Somenath Mitra, Frank A. Witzmann

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

To assess effects of carbon nanoparticle (CNP) exposure on renal epithelial cells, fullerenes (C60), single-walled carbon nanotubes (SWNT), and multi-walled carbon nanotubes (MWNT) were incubated with a confluent renal epithelial line for 48 h. At low concentrations, CNP-treated cells exhibited significant decreases in transepithelial electrical resistance (TEER) but no changes in hormone-stimulated ion transport or CNP-induced toxicity or stress responses as measured by lactate dehydrogenase or cytokine release. The changes in TEER, manifested as an inverse relationship with CNP concentration, were mirrored by an inverse correlation between dose and changes in protein expression. Lower, more physiologically relevant, concentrations of CNP have the most profound effects on barrier cell function and protein expression. These results indicate an impact of CNPs on renal epithelial cells at concentrations lower than have been previously studied and suggest caution with regard to increasing CNP levels entering the food chain due to increasing environmental pollution.

Original languageEnglish (US)
Pages (from-to)354-371
Number of pages18
JournalNanotoxicology
Volume5
Issue number3
DOIs
StatePublished - Sep 1 2011

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Nanoparticles
Carbon
Epithelial Cells
Proteins
Kidney
Carbon Nanotubes
Acoustic impedance
Electric Impedance
Environmental Pollution
Food Chain
Hormones
Ion Transport
Single-walled carbon nanotubes (SWCN)
Fullerenes
L-Lactate Dehydrogenase
Toxicity
Carbon nanotubes
Pollution
Cells
Ions

Keywords

  • Carbon nanotubes
  • Cortical collecting duct
  • Dielectric spectroscopy
  • Fullerene
  • Proteomics
  • Transepithelial resistance

ASJC Scopus subject areas

  • Biomedical Engineering
  • Toxicology

Cite this

Effect of carbon nanoparticles on renal epithelial cell structure, barrier function, and protein expression. / Blazer-Yost, Bonnie L.; Banga, Amiraj; Amos, Adam; Chernoff, Ellen; Lai, Xianyin; Li, Cheng; Mitra, Somenath; Witzmann, Frank A.

In: Nanotoxicology, Vol. 5, No. 3, 01.09.2011, p. 354-371.

Research output: Contribution to journalArticle

Blazer-Yost, Bonnie L. ; Banga, Amiraj ; Amos, Adam ; Chernoff, Ellen ; Lai, Xianyin ; Li, Cheng ; Mitra, Somenath ; Witzmann, Frank A. / Effect of carbon nanoparticles on renal epithelial cell structure, barrier function, and protein expression. In: Nanotoxicology. 2011 ; Vol. 5, No. 3. pp. 354-371.
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