Effect of cholinergic agonists on muscle from rodent proximal and distal small intestine

Thomas Nowak, B. Harrington

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Proximal and distal rat small intestine was cut into strips measuring 6.0 x 10.0 mm. Strips cut along the oral-caudal axis were called longitudinal strips, whereas those cut 90° to that axis were called circular strips. Stress in circular and longitudinal muscle strips was measured continuously as they were superfused with acetylcholine, carbamylcholine, methacholine, bethanechol, or physostigmine. Resting stress during stretch, acetylcholine-stimulated active stress, and total stress were determined. Proximal circular muscle was five times as sensitive to actylcholine as distal circular muscle (p <0.05); proximal longitudinal muscle was 2.8 times as sensitive to bethanechol as distal muscle (p <0.05). Resting, active, and total stress were similar in proximal and distal muscle, but circular muscle showed nearly twice the resting stress of longitudinal muscle at either proximal or distal sites (p <0.05). Physostigmine (10-6 M) increased acetylcholine-stimulated active stress in proximal and distal circular muscle by 29% and 70%, respectively (p <0.05), but not in longitudinal muscle (p > 0.05). This difference between proximal and distal circular muscle (41%) was also significant (p <0.05). Thus, the proximal and distal muscle of the rat small intestine differs in its sensitivity to various cholinergic agonists, but not in its length-stress properties.

Original languageEnglish (US)
Pages (from-to)1118-1125
Number of pages8
JournalGastroenterology
Volume88
Issue number5 I
StatePublished - 1985
Externally publishedYes

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Cholinergic Agonists
Small Intestine
Rodentia
Muscles
Acetylcholine
Bethanechol
Physostigmine
Methacholine Chloride
Carbachol

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Effect of cholinergic agonists on muscle from rodent proximal and distal small intestine. / Nowak, Thomas; Harrington, B.

In: Gastroenterology, Vol. 88, No. 5 I, 1985, p. 1118-1125.

Research output: Contribution to journalArticle

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AB - Proximal and distal rat small intestine was cut into strips measuring 6.0 x 10.0 mm. Strips cut along the oral-caudal axis were called longitudinal strips, whereas those cut 90° to that axis were called circular strips. Stress in circular and longitudinal muscle strips was measured continuously as they were superfused with acetylcholine, carbamylcholine, methacholine, bethanechol, or physostigmine. Resting stress during stretch, acetylcholine-stimulated active stress, and total stress were determined. Proximal circular muscle was five times as sensitive to actylcholine as distal circular muscle (p <0.05); proximal longitudinal muscle was 2.8 times as sensitive to bethanechol as distal muscle (p <0.05). Resting, active, and total stress were similar in proximal and distal muscle, but circular muscle showed nearly twice the resting stress of longitudinal muscle at either proximal or distal sites (p <0.05). Physostigmine (10-6 M) increased acetylcholine-stimulated active stress in proximal and distal circular muscle by 29% and 70%, respectively (p <0.05), but not in longitudinal muscle (p > 0.05). This difference between proximal and distal circular muscle (41%) was also significant (p <0.05). Thus, the proximal and distal muscle of the rat small intestine differs in its sensitivity to various cholinergic agonists, but not in its length-stress properties.

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