Effect of chronic graft-versus-host disease on the intestine in adult BDF1 mice

B. De Geus, H. Hogenesch, E. De Heer, J. A. Bruijn, M. Van Den Enden, J. Rozing

Research output: Contribution to journalArticle


This study was performed to characterize the intestinal lesions in chronic graft-versus-host disease (GVHD) in mice and to determine a possible role of intestinal intraepithelial lymphocytes (iIEL) in the development of these lesions. Chronic GVHD was induced by transfer of DBA/2 lymphocytes into non-irradiated (C57BL/10 x DBA/2)F1 (BDF1) recipients. There was mild to moderate mucosal oedema with multifocal mixed inflammatory cell infiltrations in the small intestine. The caecum was more severely affected with severe oedema, progressive loss of crypts and severe distortion of the mucosal architecture. The total number of iIEL did not change during the development of chronic GVHD, but there were alterations in the composition of the iIEL population. An increase of CD3+, Thy-1+ cells was accompanied by an increase of TCRαβ+ cells and a decrease of TCRγδ+ cells. There was no evidence of infiltration of donor lymphocytes into the intestinal epithelium as determined by the H2K haplotype of the iIEL. These lesions differ from previously described models of chronic GVHD, induced by DBA/2 donor lymphocytes in BDF1 recipients. We suggest that the haemopoietic organs that are used as the source of donor lymphocytes determine the outcome of the GVHD. Modulation of the composition of the donor lymphocyte population may be useful in the establishment of relevant animal models of human enteropathy.

Original languageEnglish (US)
Pages (from-to)371-377
Number of pages7
JournalInternational Journal of Experimental Pathology
Issue number4
StatePublished - Jan 1 1993
Externally publishedYes


  • animal model
  • chronic graft-versus-host disease
  • intestine
  • intraepithelial lymphocytes

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Molecular Biology
  • Cell Biology

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  • Cite this

    De Geus, B., Hogenesch, H., De Heer, E., Bruijn, J. A., Van Den Enden, M., & Rozing, J. (1993). Effect of chronic graft-versus-host disease on the intestine in adult BDF1 mice. International Journal of Experimental Pathology, 74(4), 371-377.