Effect of desensitization in solid organ transplant recipients depends on some cytokines genes polymorphism

A. L. Lobashevsky, J. E. Manwaring, M. M. Travis, B. L. Nord, N. G. Higgins, Y. A. Serov, T. S. Arnoff, G. A. Hommel-Berrey, W. C. Goggins, Tim Taber, C. B. Carter, Daniel Smith, T. C. Wozniak, Jacqueline O'Donnell, Mark Turrentine

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Desensitization (DS) is widely used to decrease PRA in solid organs transplant candidates (TC). Various numbers of cycles of DS are required to reduce or eliminate donor specific antibodies (DSA). The goal of this study was to investigate if there was a correlation between polymorphism (PM) of some cytokine genes and intensity of DS required to make the recipient/donor cross match compatible. Thirty-one TCs were included in the study. Antibody specificity, percent of reactive antibodies (PRA) and serum concentration of cytokines were analyzed using the LUMINEX platform. PCR-SSP method was used for IL-1α, IL-1β, IL-1R, IL-1Rα, IL-4Rα, IL-12, IFNγ, TGFβ1, TNFα, IL-2, IL-4, IL-6 and IL-10 gene PM analysis. Significant relationship between PM of genes encoding IL-4Rα, IFNγ and IL-12 (p70) and susceptibility to DS was demonstrated (p = 0.04, p = 0.01 and p = 0.05 respectively). Correlation between elevated serum level of IL-12 (p70) and A/A or C/A genotype at - 1188 position was found in resistant to DS TCs (p = 0.015). These results indicate that analysis PM of genes encoding IL-4R, IFNγ and IL-12 enables to define the DS strategy in TCs more accurately regarding the number of plasmapheresis (PP) cycles and dose of intravenous immunoglobulin (IVIG).

Original languageEnglish
Pages (from-to)169-178
Number of pages10
JournalTransplant Immunology
Volume21
Issue number3
DOIs
StatePublished - Jul 2009

Fingerprint

Interleukin-12
Cytokines
Transplants
Interleukin-1
Genes
Antibodies
Tissue Donors
Plasmapheresis
Antibody Specificity
Intravenous Immunoglobulins
Serum
Interleukin-4
Interleukin-10
Interleukin-2
Interleukin-6
Genotype
Polymerase Chain Reaction
Transplant Recipients

Keywords

  • Cytokines
  • Desensitization
  • Genomic polymorphism
  • High PRA

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy
  • Transplantation

Cite this

Effect of desensitization in solid organ transplant recipients depends on some cytokines genes polymorphism. / Lobashevsky, A. L.; Manwaring, J. E.; Travis, M. M.; Nord, B. L.; Higgins, N. G.; Serov, Y. A.; Arnoff, T. S.; Hommel-Berrey, G. A.; Goggins, W. C.; Taber, Tim; Carter, C. B.; Smith, Daniel; Wozniak, T. C.; O'Donnell, Jacqueline; Turrentine, Mark.

In: Transplant Immunology, Vol. 21, No. 3, 07.2009, p. 169-178.

Research output: Contribution to journalArticle

Lobashevsky, AL, Manwaring, JE, Travis, MM, Nord, BL, Higgins, NG, Serov, YA, Arnoff, TS, Hommel-Berrey, GA, Goggins, WC, Taber, T, Carter, CB, Smith, D, Wozniak, TC, O'Donnell, J & Turrentine, M 2009, 'Effect of desensitization in solid organ transplant recipients depends on some cytokines genes polymorphism', Transplant Immunology, vol. 21, no. 3, pp. 169-178. https://doi.org/10.1016/j.trim.2009.03.002
Lobashevsky, A. L. ; Manwaring, J. E. ; Travis, M. M. ; Nord, B. L. ; Higgins, N. G. ; Serov, Y. A. ; Arnoff, T. S. ; Hommel-Berrey, G. A. ; Goggins, W. C. ; Taber, Tim ; Carter, C. B. ; Smith, Daniel ; Wozniak, T. C. ; O'Donnell, Jacqueline ; Turrentine, Mark. / Effect of desensitization in solid organ transplant recipients depends on some cytokines genes polymorphism. In: Transplant Immunology. 2009 ; Vol. 21, No. 3. pp. 169-178.
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AU - Nord, B. L.

AU - Higgins, N. G.

AU - Serov, Y. A.

AU - Arnoff, T. S.

AU - Hommel-Berrey, G. A.

AU - Goggins, W. C.

AU - Taber, Tim

AU - Carter, C. B.

AU - Smith, Daniel

AU - Wozniak, T. C.

AU - O'Donnell, Jacqueline

AU - Turrentine, Mark

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N2 - Desensitization (DS) is widely used to decrease PRA in solid organs transplant candidates (TC). Various numbers of cycles of DS are required to reduce or eliminate donor specific antibodies (DSA). The goal of this study was to investigate if there was a correlation between polymorphism (PM) of some cytokine genes and intensity of DS required to make the recipient/donor cross match compatible. Thirty-one TCs were included in the study. Antibody specificity, percent of reactive antibodies (PRA) and serum concentration of cytokines were analyzed using the LUMINEX platform. PCR-SSP method was used for IL-1α, IL-1β, IL-1R, IL-1Rα, IL-4Rα, IL-12, IFNγ, TGFβ1, TNFα, IL-2, IL-4, IL-6 and IL-10 gene PM analysis. Significant relationship between PM of genes encoding IL-4Rα, IFNγ and IL-12 (p70) and susceptibility to DS was demonstrated (p = 0.04, p = 0.01 and p = 0.05 respectively). Correlation between elevated serum level of IL-12 (p70) and A/A or C/A genotype at - 1188 position was found in resistant to DS TCs (p = 0.015). These results indicate that analysis PM of genes encoding IL-4R, IFNγ and IL-12 enables to define the DS strategy in TCs more accurately regarding the number of plasmapheresis (PP) cycles and dose of intravenous immunoglobulin (IVIG).

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