Effect of developmental stage of HSC and recipient on transplant outcomes

Natasha Arora, Pamela L. Wenzel, Shannon L. McKinney-Freeman, Samantha J. Ross, Peter G. Kim, Stephanie S. Chou, Momoko Yoshimoto, Mervin Yoder, George Q. Daley

Research output: Contribution to journalArticle

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Abstract

The first hematopoietic stem cells (HSCs) that engraft irradiated adult mice arise in the aorta-gonad-mesonephros (AGM) on embryonic day 11.5 (E11.5). However, at this stage, there is a discrepancy between the apparent frequency of HSCs depicted with imaging and their rarity when measured with limiting dilution transplant. We have attempted to reconcile this difference using neonatal recipients, which are more permissive for embryonic HSC engraftment. We found that embryonic HSCs from E9.5 and E10.5 preferentially engrafted neonates, whereas developmentally mature, definitive HSCs from E14.5 fetal liver or adult bone marrow (BM) more robustly engrafted adults. Neonatal engraftment was enhanced after treating adult BM-derived HSCs with interferon. Adult BM-derived HSCs preferentially homed to theliver in neonatal mice yet showed balanced homing to the liver and spleen in adults. These findings emphasize the functional differences between nascent and mature definitive HSCs.

Original languageEnglish
Pages (from-to)621-628
Number of pages8
JournalDevelopmental Cell
Volume29
Issue number5
DOIs
StatePublished - Jun 9 2014

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Transplants
Hematopoietic Stem Cells
Stem cells
Bone
Bone Marrow
Embryonic Stem Cells
Liver
Mesonephros
Transplant Recipients
Gonads
Interferons
Dilution
Aorta
Spleen
Newborn Infant
Imaging techniques

ASJC Scopus subject areas

  • Developmental Biology

Cite this

Arora, N., Wenzel, P. L., McKinney-Freeman, S. L., Ross, S. J., Kim, P. G., Chou, S. S., ... Daley, G. Q. (2014). Effect of developmental stage of HSC and recipient on transplant outcomes. Developmental Cell, 29(5), 621-628. https://doi.org/10.1016/j.devcel.2014.04.013

Effect of developmental stage of HSC and recipient on transplant outcomes. / Arora, Natasha; Wenzel, Pamela L.; McKinney-Freeman, Shannon L.; Ross, Samantha J.; Kim, Peter G.; Chou, Stephanie S.; Yoshimoto, Momoko; Yoder, Mervin; Daley, George Q.

In: Developmental Cell, Vol. 29, No. 5, 09.06.2014, p. 621-628.

Research output: Contribution to journalArticle

Arora, N, Wenzel, PL, McKinney-Freeman, SL, Ross, SJ, Kim, PG, Chou, SS, Yoshimoto, M, Yoder, M & Daley, GQ 2014, 'Effect of developmental stage of HSC and recipient on transplant outcomes', Developmental Cell, vol. 29, no. 5, pp. 621-628. https://doi.org/10.1016/j.devcel.2014.04.013
Arora N, Wenzel PL, McKinney-Freeman SL, Ross SJ, Kim PG, Chou SS et al. Effect of developmental stage of HSC and recipient on transplant outcomes. Developmental Cell. 2014 Jun 9;29(5):621-628. https://doi.org/10.1016/j.devcel.2014.04.013
Arora, Natasha ; Wenzel, Pamela L. ; McKinney-Freeman, Shannon L. ; Ross, Samantha J. ; Kim, Peter G. ; Chou, Stephanie S. ; Yoshimoto, Momoko ; Yoder, Mervin ; Daley, George Q. / Effect of developmental stage of HSC and recipient on transplant outcomes. In: Developmental Cell. 2014 ; Vol. 29, No. 5. pp. 621-628.
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