Effect of different endodontic regeneration protocols on wettability, roughness, and chemical composition of surface dentin

Ghaeth H. Yassen, Alaa H.A. Sabrah, George J. Eckert, Jeffrey A. Platt

Research output: Contribution to journalArticle

24 Scopus citations


Introduction We investigated the changes in physiochemical properties of dentin surfaces after performing different endodontic regeneration protocols. Methods Human dentin slices were randomized into 4 treatment groups and 1 untreated control group (n = 10). One treatment group was irrigated with sodium hypochlorite (NaOCl) for 5 minutes followed by EDTA for 10 minutes. The other 3 treatment groups were irrigated with NaOCl; treated for 4 weeks with triple antibiotic paste (TAP), diluted triple antibiotic paste (DTAP), or calcium hydroxide (Ca[OH]<inf>2</inf>); and then irrigated with EDTA. After treatment, contact angles between a blood analog and dentin surfaces were evaluated. Surface roughness and chemical composition were characterized using optical profilometry and energy-dispersive X-ray spectroscopy, respectively. One-way analysis of variance followed by Fisher least significant difference tests were used for statistical analyses. Results All treatment groups showed a significant reduction in wettability and a significant increase in surface roughness when compared with untreated dentin. Dentin treated with Ca(OH)<inf>2</inf> had significantly lower wettability compared with all other groups. No significant difference in wettability was found between dentin treated with DTAP and TAP protocols. Dentin treated with TAP had significantly higher surface roughness compared with all other groups. Untreated dentin and NaOCl + EDTA-treated dentin had significantly higher calcium and phosphorus as well as significantly lower carbon compared with dentin treated with Ca(OH)<inf>2</inf>, DTAP, and TAP. Conclusions Endodontic regeneration protocols had a significant effect on wettability, surface roughness, and chemical composition of surface dentin. The Ca(OH)<inf>2</inf> protocol caused a significant reduction in dentin wettability compared with TAP or DTAP protocols.

Original languageEnglish (US)
Pages (from-to)956-960
Number of pages5
JournalJournal of endodontics
Issue number6
StatePublished - Jun 1 2015


  • Calcium hydroxide
  • dentin
  • endodontic regeneration
  • surface roughness
  • triple antibiotic paste
  • wettability

ASJC Scopus subject areas

  • Dentistry(all)

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