Effect of different obesogenic diets on pancreatic histology in Ossabaw miniature swine

Allison M. Fullenkamp, Lauren N. Bell, Reiesha D. Robbins, Lydia Lee, Romil Saxena, Mouhamad Alloosh, James E. Klaunig, Raghavendra G. Mirmira, Michael Sturek, Naga Chalasani

Research output: Contribution to journalArticlepeer-review

13 Scopus citations


OBJECTIVE: Obesity is a factor in the outcome and severity of pancreatic conditions. We examined the effect of hypercaloric diets on the pancreata of Ossabaw swine, a large animal model of metabolic syndrome and obesity. METHODS: Swine were fed with 1 of 4 diets: high-fructose (n = 9), atherogenic (n = 10), modified atherogenic (n = 6), or eucaloric standard diet (n = 12) for 24 weeks. Serum chemistries were measured, and pancreata were examined for histological abnormalities including steatosis, inflammation or fibrosis, insulin content, and oxidative stress. RESULTS: The fructose, atherogenic, and modified atherogenic diet groups exhibited obesity, metabolic syndrome, islet enlargement, and significantly increased pancreatic steatosis (22.9% ± 7.5%, 19.7% ± 7.7%, and 38.7% ± 15.3% fat in total tissue area, respectively) compared with controls (9.3% ± 1.9%; P < 0.05). The modified atherogenic diet group showed significantly increased oxidative stress levels as evidenced by elevated serum malondialdehyde (3.0 ± 3.3 vs 1.5 ± 0.3 μmol/L in controls; P = 0.006) and pancreatic malondialdehyde (0.1 ± 0.12 vs 0.04 ± 0.01 nmol/mg protein in controls; P = 0.01). None of the swine exhibited pancreatitis or cellular injury. CONCLUSIONS: Ossabaw swine fed with a modified atherogenic diet developed significant pancreatic steatosis and increased oxidative stress, but no other histological abnormalities were observed.

Original languageEnglish (US)
Pages (from-to)438-443
Number of pages6
Issue number3
StatePublished - Apr 2011


  • animal model
  • fatty liver
  • fatty pancreas
  • metabolic syndrome

ASJC Scopus subject areas

  • Hepatology
  • Internal Medicine
  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Fingerprint Dive into the research topics of 'Effect of different obesogenic diets on pancreatic histology in Ossabaw miniature swine'. Together they form a unique fingerprint.

Cite this