Effect of disuse on sarcoplasmic reticulum in fast and slow skeletal muscle.

D. H. Kim, Frank Witzmann, R. H. Fitts

Research output: Contribution to journalArticle

Abstract

The effect of 6 wk of hindlimb immobilization on rat skeletal muscle sarcoplasmic reticulum (SR) was determined in the slow-twitch, type I soleus (SOL), the fast-twitch, type IIA deep region of the vastus lateralis (DVL), and the fast-twitch, type IIB superficial region of the vastus lateralis (SVL). Immobilization produced a significant decline in the Ca2+ uptake rate (Vmax) of SR vesicles from the slow SOL (0.930 +/- 0.116 to 0.365 +/- 0.071 mumol Ca2+ . mg-1 . min-1), while the SR Vmax increased in the fast SVL (2.763 +/- 0.133 to 5.209 +/- 0.687) and was unaltered in the DVL. Vesicles from the fast SVL and DVL also exhibited a higher total Ca2+ uptake capacity following immobilization. An evaluation of the time course of the immobilization-mediated effect revealed an increased Ca2+ uptake capacity in all three samples after 1 wk. In the SOL total Ca2+ uptake returned to control level after 2 wk, while in the fast-twitch muscles the higher capacities were maintained. The Ca2+-stimulated SR ATPase activity was not altered in any of the muscles studies, although the total SR ATPase activity increased twofold in the slow SOL.

Original languageEnglish
JournalThe American journal of physiology
Volume243
Issue number3
StatePublished - Sep 1982
Externally publishedYes

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Quadriceps Muscle
Sarcoplasmic Reticulum
Skeletal Muscle
Immobilization
Adenosine Triphosphatases
Hindlimb Suspension
Muscles

ASJC Scopus subject areas

  • Medicine(all)

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Effect of disuse on sarcoplasmic reticulum in fast and slow skeletal muscle. / Kim, D. H.; Witzmann, Frank; Fitts, R. H.

In: The American journal of physiology, Vol. 243, No. 3, 09.1982.

Research output: Contribution to journalArticle

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