Effect of disuse on sarcoplasmic reticulum in fast and slow skeletal muscle

D. H. Kim, Frank Witzmann, R. H. Fitts

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

The effect of 6 wk of hindlimb immobilization on rat skeletal muscle sarcoplasmic reticulum (SR) was determined in the slow-twitch, type I soleus (SOL), the fast-twitch, type IIA deep region of the vastus lateralis (DVL), and the fast-twitch, type IIB superficial region of the vastus lateralis (SVL). Immobilization produced a significant decline in the Ca2+ uptake rate (V(max)) of SR vesicles from the slow SOL (0.930 ± 0.116 to 0.365 ± 0.071 μmol Ca2+.mg-1.min-1), while the SR V(max) increased in the fast SVL (2.763 ± 0.133 to 5.209 ± 0.687) and was unaltered in the DVL. Vesicles from the fast SVL and DVL also exhibited a higher total Ca2+ uptake capacity following immobilization. An evaluation of the time course of the immobilization-mediated effect revealed an increased Ca2+ uptake capacity in all three samples after 1 wk. In the SOL total Ca2+ uptake returned to control level after 2 wk, while inthe fast-twitch muscles the higher capacities were maintained. The Ca2+-stimulated SR ATPase activity was not altered in any of the muscle studies, although the total SR ATPase activity increased twofold in the slow SOL.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Cell Physiology
Volume12
Issue number2
StatePublished - 1982
Externally publishedYes

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Quadriceps Muscle
Sarcoplasmic Reticulum
Muscle
Skeletal Muscle
Adenosine Triphosphatases
Immobilization
Level control
Rats
Hindlimb Suspension
Muscles

ASJC Scopus subject areas

  • Cell Biology
  • Clinical Biochemistry
  • Physiology

Cite this

Effect of disuse on sarcoplasmic reticulum in fast and slow skeletal muscle. / Kim, D. H.; Witzmann, Frank; Fitts, R. H.

In: American Journal of Physiology - Cell Physiology, Vol. 12, No. 2, 1982.

Research output: Contribution to journalArticle

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