Effect of disuse on sarcoplasmic reticulum in fast and slow skeletal muscle

D. H. Kim, F. A. Witzmann, R. H. Fitts

Research output: Contribution to journalArticle

32 Scopus citations

Abstract

The effect of 6 wk of hindlimb immobilization on rat skeletal muscle sarcoplasmic reticulum (SR) was determined in the slow-twitch, type I soleus (SOL), the fast-twitch, type IIA deep region of the vastus lateralis (DVL), and the fast-twitch, type IIB superficial region of the vastus lateralis (SVL). Immobilization produced a significant decline in the Ca2+ uptake rate (V(max)) of SR vesicles from the slow SOL (0.930 ± 0.116 to 0.365 ± 0.071 μmol Ca2+.mg-1.min-1), while the SR V(max) increased in the fast SVL (2.763 ± 0.133 to 5.209 ± 0.687) and was unaltered in the DVL. Vesicles from the fast SVL and DVL also exhibited a higher total Ca2+ uptake capacity following immobilization. An evaluation of the time course of the immobilization-mediated effect revealed an increased Ca2+ uptake capacity in all three samples after 1 wk. In the SOL total Ca2+ uptake returned to control level after 2 wk, while inthe fast-twitch muscles the higher capacities were maintained. The Ca2+-stimulated SR ATPase activity was not altered in any of the muscle studies, although the total SR ATPase activity increased twofold in the slow SOL.

Original languageEnglish (US)
Pages (from-to)C156-C160
JournalAmerican Journal of Physiology - Cell Physiology
Volume12
Issue number2
DOIs
StatePublished - 1982

ASJC Scopus subject areas

  • Physiology
  • Cell Biology

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