Effect of forskolin on collagen production in clonal osteoblastic MC3T3-E1 cells

Yoshiyuki Hakeda, Yoshinori Nakatani, Takahiko Yoshino, Noriyoshi Kurihara, Kuniya Fujita, Norihiko Maeda, Masayoshi Kumegawa

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The effect of forskolin on collagen production in osteoblasts was investigated by using clonal osteoblastic MC3T3-E1 cells cultured in a-minimum essential medium containing 0.1% bovine serum albumin. Forskolin increased the adenylate cyclase activity in membranes pelleted from homogenates of the cell line in a dose-dependent manner. The drug caused a 13-fold stimulation at 10-4 M, indicating that the compound directly acts on adenylate cyclase, leading to an increase in the intracellular cAMP content of the cells. Collagen accumulation in the cultures was elevated by one-day treatment with 5 × 10-5 M forskolin to about twice that in the controls. The stimulation was mainly due to an elevation in collagen synthesis but not to an inhibition of intracellular collagen degradation because forskolin dose-dependently increased collagen synthesis; it also significantly increased the amount of low-molecular-weight hydroxyproline found in the cultures. Cells treated with forskolin produced mainly type I collagen, as found in bone matrix in situ, with only small amounts of other types of collagen. Furthermore, forskolin time-dependently inhibited DNA synthesis in the cells, indicating that the increase in type I collagen synthesis by forskolin was not due to stimulated cell proliferation. These results suggest that cAMP is closely linked to the differentiation of osteoblasts in vitro.

Original languageEnglish (US)
Pages (from-to)1463-1469
Number of pages7
JournalJournal of Biochemistry
Volume101
Issue number6
StatePublished - 1987
Externally publishedYes

Fingerprint

Collagen
Colforsin
Cell
Synthesis
Osteoblasts
Collagen Type I
Adenylyl Cyclases
Dose
Hydroxyproline
Bone Matrix
Cell proliferation
Cultured Cells
Bovine Serum Albumin
Cell Proliferation
Bone
Molecular Weight
Molecular weight
Cells
Drugs
Degradation

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Physiology (medical)
  • Radiology Nuclear Medicine and imaging
  • Molecular Biology
  • Biochemistry

Cite this

Hakeda, Y., Nakatani, Y., Yoshino, T., Kurihara, N., Fujita, K., Maeda, N., & Kumegawa, M. (1987). Effect of forskolin on collagen production in clonal osteoblastic MC3T3-E1 cells. Journal of Biochemistry, 101(6), 1463-1469.

Effect of forskolin on collagen production in clonal osteoblastic MC3T3-E1 cells. / Hakeda, Yoshiyuki; Nakatani, Yoshinori; Yoshino, Takahiko; Kurihara, Noriyoshi; Fujita, Kuniya; Maeda, Norihiko; Kumegawa, Masayoshi.

In: Journal of Biochemistry, Vol. 101, No. 6, 1987, p. 1463-1469.

Research output: Contribution to journalArticle

Hakeda, Y, Nakatani, Y, Yoshino, T, Kurihara, N, Fujita, K, Maeda, N & Kumegawa, M 1987, 'Effect of forskolin on collagen production in clonal osteoblastic MC3T3-E1 cells', Journal of Biochemistry, vol. 101, no. 6, pp. 1463-1469.
Hakeda Y, Nakatani Y, Yoshino T, Kurihara N, Fujita K, Maeda N et al. Effect of forskolin on collagen production in clonal osteoblastic MC3T3-E1 cells. Journal of Biochemistry. 1987;101(6):1463-1469.
Hakeda, Yoshiyuki ; Nakatani, Yoshinori ; Yoshino, Takahiko ; Kurihara, Noriyoshi ; Fujita, Kuniya ; Maeda, Norihiko ; Kumegawa, Masayoshi. / Effect of forskolin on collagen production in clonal osteoblastic MC3T3-E1 cells. In: Journal of Biochemistry. 1987 ; Vol. 101, No. 6. pp. 1463-1469.
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