Effect of High-Calcium Diet on Coronary Artery Disease in Ossabaw Miniature Swine With Metabolic Syndrome

Alyssa K. Phillips-Eakley, Mikaela L. McKenney-Drake, Martin Bahls, Sean C. Newcomer, John S. Radcliffe, Meryl E. Wastney, William G. Van Alstine, George Jackson, Mouhamad Alloosh, Berdine R. Martin, Michael Sturek, Connie M. Weaver

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

BACKGROUND: Calcium is a shortfall essential nutrient that has been a mainstay of osteoporosis management. Recent and limited findings have prompted concern about the contribution of calcium supplementation to cardiovascular risk. A proposed mechanism is through the acceleration of coronary artery calcification. Determining causality between calcium intake and coronary artery calcification has been hindered by a lack of sensitive methodology to monitor early vascular calcium accumulation. The primary study aim was to assess the impact of high calcium intake on coronary artery calcification using innovative calcium tracer kinetic modeling in Ossabaw swine with diet-induced metabolic syndrome. Secondary end points (in vitro wire myography, histopathology, intravascular ultrasound) assessed coronary disease.

METHODS AND RESULTS: Pigs (n=24; aged ≈15 months) were fed an atherogenic diet with adequate calcium (0.33% by weight) or high calcium (1.90% from calcium carbonate or dairy) for 6 months. Following 5 months of feeding, all pigs were dosed intravenously with (41)Ca, a rare isotope that can be measured in serum and tissues at a sensitivity of 10(-18) mol/L by accelerator mass spectrometry. Kinetic modeling evaluated early coronary artery calcification using (41)Ca values measured in serial blood samples (collected over 27 days) and coronary artery samples obtained at sacrifice. Serum disappearance of (41)Ca and total coronary artery (41)Ca accumulation did not differ among groups. Secondary end points demonstrated no treatment differences in coronary artery disease or function.

CONCLUSION: There was no detectable effect of high calcium diets (from dairy or calcium carbonate) on coronary artery calcium deposition in metabolic syndrome swine.

Original languageEnglish (US)
Pages (from-to)e001620
JournalJournal of the American Heart Association
Volume4
Issue number8
DOIs
StatePublished - Aug 13 2015

Fingerprint

Miniature Swine
Coronary Artery Disease
Diet
Calcium
Coronary Vessels
Swine
Calcium Carbonate
Myography
Atherogenic Diet
Serum
Isotopes
Causality
Osteoporosis
Coronary Disease
Blood Vessels
Mass Spectrometry

Keywords

  • calcium
  • coronary disease
  • diet
  • isotopes
  • nutrition

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Phillips-Eakley, A. K., McKenney-Drake, M. L., Bahls, M., Newcomer, S. C., Radcliffe, J. S., Wastney, M. E., ... Weaver, C. M. (2015). Effect of High-Calcium Diet on Coronary Artery Disease in Ossabaw Miniature Swine With Metabolic Syndrome. Journal of the American Heart Association, 4(8), e001620. https://doi.org/10.1161/JAHA.114.001620

Effect of High-Calcium Diet on Coronary Artery Disease in Ossabaw Miniature Swine With Metabolic Syndrome. / Phillips-Eakley, Alyssa K.; McKenney-Drake, Mikaela L.; Bahls, Martin; Newcomer, Sean C.; Radcliffe, John S.; Wastney, Meryl E.; Van Alstine, William G.; Jackson, George; Alloosh, Mouhamad; Martin, Berdine R.; Sturek, Michael; Weaver, Connie M.

In: Journal of the American Heart Association, Vol. 4, No. 8, 13.08.2015, p. e001620.

Research output: Contribution to journalArticle

Phillips-Eakley, AK, McKenney-Drake, ML, Bahls, M, Newcomer, SC, Radcliffe, JS, Wastney, ME, Van Alstine, WG, Jackson, G, Alloosh, M, Martin, BR, Sturek, M & Weaver, CM 2015, 'Effect of High-Calcium Diet on Coronary Artery Disease in Ossabaw Miniature Swine With Metabolic Syndrome', Journal of the American Heart Association, vol. 4, no. 8, pp. e001620. https://doi.org/10.1161/JAHA.114.001620
Phillips-Eakley, Alyssa K. ; McKenney-Drake, Mikaela L. ; Bahls, Martin ; Newcomer, Sean C. ; Radcliffe, John S. ; Wastney, Meryl E. ; Van Alstine, William G. ; Jackson, George ; Alloosh, Mouhamad ; Martin, Berdine R. ; Sturek, Michael ; Weaver, Connie M. / Effect of High-Calcium Diet on Coronary Artery Disease in Ossabaw Miniature Swine With Metabolic Syndrome. In: Journal of the American Heart Association. 2015 ; Vol. 4, No. 8. pp. e001620.
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abstract = "BACKGROUND: Calcium is a shortfall essential nutrient that has been a mainstay of osteoporosis management. Recent and limited findings have prompted concern about the contribution of calcium supplementation to cardiovascular risk. A proposed mechanism is through the acceleration of coronary artery calcification. Determining causality between calcium intake and coronary artery calcification has been hindered by a lack of sensitive methodology to monitor early vascular calcium accumulation. The primary study aim was to assess the impact of high calcium intake on coronary artery calcification using innovative calcium tracer kinetic modeling in Ossabaw swine with diet-induced metabolic syndrome. Secondary end points (in vitro wire myography, histopathology, intravascular ultrasound) assessed coronary disease.METHODS AND RESULTS: Pigs (n=24; aged ≈15 months) were fed an atherogenic diet with adequate calcium (0.33{\%} by weight) or high calcium (1.90{\%} from calcium carbonate or dairy) for 6 months. Following 5 months of feeding, all pigs were dosed intravenously with (41)Ca, a rare isotope that can be measured in serum and tissues at a sensitivity of 10(-18) mol/L by accelerator mass spectrometry. Kinetic modeling evaluated early coronary artery calcification using (41)Ca values measured in serial blood samples (collected over 27 days) and coronary artery samples obtained at sacrifice. Serum disappearance of (41)Ca and total coronary artery (41)Ca accumulation did not differ among groups. Secondary end points demonstrated no treatment differences in coronary artery disease or function.CONCLUSION: There was no detectable effect of high calcium diets (from dairy or calcium carbonate) on coronary artery calcium deposition in metabolic syndrome swine.",
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AU - Phillips-Eakley, Alyssa K.

AU - McKenney-Drake, Mikaela L.

AU - Bahls, Martin

AU - Newcomer, Sean C.

AU - Radcliffe, John S.

AU - Wastney, Meryl E.

AU - Van Alstine, William G.

AU - Jackson, George

AU - Alloosh, Mouhamad

AU - Martin, Berdine R.

AU - Sturek, Michael

AU - Weaver, Connie M.

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N2 - BACKGROUND: Calcium is a shortfall essential nutrient that has been a mainstay of osteoporosis management. Recent and limited findings have prompted concern about the contribution of calcium supplementation to cardiovascular risk. A proposed mechanism is through the acceleration of coronary artery calcification. Determining causality between calcium intake and coronary artery calcification has been hindered by a lack of sensitive methodology to monitor early vascular calcium accumulation. The primary study aim was to assess the impact of high calcium intake on coronary artery calcification using innovative calcium tracer kinetic modeling in Ossabaw swine with diet-induced metabolic syndrome. Secondary end points (in vitro wire myography, histopathology, intravascular ultrasound) assessed coronary disease.METHODS AND RESULTS: Pigs (n=24; aged ≈15 months) were fed an atherogenic diet with adequate calcium (0.33% by weight) or high calcium (1.90% from calcium carbonate or dairy) for 6 months. Following 5 months of feeding, all pigs were dosed intravenously with (41)Ca, a rare isotope that can be measured in serum and tissues at a sensitivity of 10(-18) mol/L by accelerator mass spectrometry. Kinetic modeling evaluated early coronary artery calcification using (41)Ca values measured in serial blood samples (collected over 27 days) and coronary artery samples obtained at sacrifice. Serum disappearance of (41)Ca and total coronary artery (41)Ca accumulation did not differ among groups. Secondary end points demonstrated no treatment differences in coronary artery disease or function.CONCLUSION: There was no detectable effect of high calcium diets (from dairy or calcium carbonate) on coronary artery calcium deposition in metabolic syndrome swine.

AB - BACKGROUND: Calcium is a shortfall essential nutrient that has been a mainstay of osteoporosis management. Recent and limited findings have prompted concern about the contribution of calcium supplementation to cardiovascular risk. A proposed mechanism is through the acceleration of coronary artery calcification. Determining causality between calcium intake and coronary artery calcification has been hindered by a lack of sensitive methodology to monitor early vascular calcium accumulation. The primary study aim was to assess the impact of high calcium intake on coronary artery calcification using innovative calcium tracer kinetic modeling in Ossabaw swine with diet-induced metabolic syndrome. Secondary end points (in vitro wire myography, histopathology, intravascular ultrasound) assessed coronary disease.METHODS AND RESULTS: Pigs (n=24; aged ≈15 months) were fed an atherogenic diet with adequate calcium (0.33% by weight) or high calcium (1.90% from calcium carbonate or dairy) for 6 months. Following 5 months of feeding, all pigs were dosed intravenously with (41)Ca, a rare isotope that can be measured in serum and tissues at a sensitivity of 10(-18) mol/L by accelerator mass spectrometry. Kinetic modeling evaluated early coronary artery calcification using (41)Ca values measured in serial blood samples (collected over 27 days) and coronary artery samples obtained at sacrifice. Serum disappearance of (41)Ca and total coronary artery (41)Ca accumulation did not differ among groups. Secondary end points demonstrated no treatment differences in coronary artery disease or function.CONCLUSION: There was no detectable effect of high calcium diets (from dairy or calcium carbonate) on coronary artery calcium deposition in metabolic syndrome swine.

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