Effect of highly active antiretroviral therapy on viral burden in the lungs of HIV-infected subjects

Homer L. Twigg, Michael Weiden, Fred Valentine, Carol T. Schnizlein-Bick, Roland Bassett, Lu Zheng, Joseph Wheat, Richard B. Day, Helen Rominger, Ronald G. Collman, Lawrence Fox, Barbara Brizz, Joan Dragavon, Robert W. Coombs, R. Pat Bucy

Research output: Contribution to journalArticle

33 Scopus citations

Abstract

Background. Human immunodeficiency virus (HIV) is readily detectable in the lungs of infected subjects and leads to an accumulation of CD8+ lymphocytes in the alveolar space. Although highly active antiretroviral therapy (HAART) is effective in reducing viremia, less is known about its effect on tissue compartments. The AIDS Clinical Trials Group Protocol 723 Team evaluated the effect of HAART on lung viral load and cellular constituents. Methods. Bronchoalveolar lavage (BAL) fluid and blood were collected before initiation of HAART and again at 4 and 24 weeks after initiation of therapy. The BAL cell differential was determined, lymphocyte phenotyping was performed, and acellular BAL fluid, plasma HIV RNA load, and BAL cell and peripheral blood mononuclear cell HIV RNA and DNA loads were measured. Results. HAART induced a rapid decrease in HIV that was detectable in acellular BAL fluid and a slower decrease in the HIV RNA and DNA loads in BAL cells. HAART was associated with a significant decrease in the absolute number and percentage of CD8+ alveolar lymphocytes. There was a significant correlation between residual BAL cell DNA at 24 weeks and the absolute number of CD4+ lymphocytes in the alveolar space. Conclusion. HAART is associated with a significant decrease in the pulmonary HIV burden and a return of alveolar cellular constituents to normal.

Original languageEnglish (US)
Pages (from-to)109-116
Number of pages8
JournalJournal of Infectious Diseases
Volume197
Issue number1
DOIs
StatePublished - Jan 1 2008

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases

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    Twigg, H. L., Weiden, M., Valentine, F., Schnizlein-Bick, C. T., Bassett, R., Zheng, L., Wheat, J., Day, R. B., Rominger, H., Collman, R. G., Fox, L., Brizz, B., Dragavon, J., Coombs, R. W., & Bucy, R. P. (2008). Effect of highly active antiretroviral therapy on viral burden in the lungs of HIV-infected subjects. Journal of Infectious Diseases, 197(1), 109-116. https://doi.org/10.1086/523766