Effect of hydrogen peroxide on proliferation, apoptosis and interleukin-2 production of Jurkat T cells

Gabi Nindl, Nathan R. Peterson, Ellen F. Hughes, Lee R. Waite, Mary T. Johnson

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Hydrogen peroxide (H2O2) is well known as a cell damaging agent that is produced during normal cell metabolism of aerobic organisms. An excessive production of oxygen metabolites such as H 2O2 leads to oxidative stress and disease. On the other hand, it recently was discovered that H2O2 is not only a deleterious oxidant for cells but can also play an important role as a beneficial signaling molecule in certain cells such as T lymphocytes. T lymphocytes are major regulatory cells in the inflammatory cascade and can act by releasing either toxins or beneficial signaling molecules. Understanding how to regulate the actions of H2O2 in T cells will allow for the creation of novel ways to improve the treatment of inflammatory diseases. The current study presents baseline information on the effects of H 2O2 on Jurkat cells, a T cell line list we use as a T cell model for therapeutic research. We first determined the half-life of 0-80 μmolar H2O2 added to Jurkat cultures using a real-time H2O2 monitoring system. We then exposed Jurkat cells to such H2O2 concentrations and found that 50±10 μmolar H2O2 promoted interleukin-2 production in cells activated with anti-CD3 at the T cell receptor plus phorbol myristate acetate as a co-stimulatory signal. These effects were not seen in non-activated, normal Jurkat cells, where H2O2 inhibited cell proliferation and induced apoptosis in a dose-dependent way without affecting interleukin-2 production. Our data indicate that Jurkat cells can model both healthy and inflammatory T cells that respond differently to oxidative metabolites such as H2O2.

Original languageEnglish
Pages (from-to)123-128
Number of pages6
JournalBiomedical Sciences Instrumentation
Volume40
StatePublished - 2004

Fingerprint

Jurkat Cells
T-cells
Cell death
Hydrogen peroxide
Hydrogen Peroxide
Interleukin-2
Apoptosis
T-Lymphocytes
Metabolites
Therapeutic Human Experimentation
Tetradecanoylphorbol Acetate
Regulatory T-Lymphocytes
Molecules
T-Cell Antigen Receptor
Oxidative stress
Oxidants
Cell proliferation
Half-Life
Metabolism
Oxidative Stress

Keywords

  • Caspase 3
  • Cell signaling
  • Cytokine
  • Inflammation
  • Reactive oxygen species
  • ROS
  • T lymphocytes

ASJC Scopus subject areas

  • Hardware and Architecture

Cite this

Nindl, G., Peterson, N. R., Hughes, E. F., Waite, L. R., & Johnson, M. T. (2004). Effect of hydrogen peroxide on proliferation, apoptosis and interleukin-2 production of Jurkat T cells. Biomedical Sciences Instrumentation, 40, 123-128.

Effect of hydrogen peroxide on proliferation, apoptosis and interleukin-2 production of Jurkat T cells. / Nindl, Gabi; Peterson, Nathan R.; Hughes, Ellen F.; Waite, Lee R.; Johnson, Mary T.

In: Biomedical Sciences Instrumentation, Vol. 40, 2004, p. 123-128.

Research output: Contribution to journalArticle

Nindl, G, Peterson, NR, Hughes, EF, Waite, LR & Johnson, MT 2004, 'Effect of hydrogen peroxide on proliferation, apoptosis and interleukin-2 production of Jurkat T cells', Biomedical Sciences Instrumentation, vol. 40, pp. 123-128.
Nindl, Gabi ; Peterson, Nathan R. ; Hughes, Ellen F. ; Waite, Lee R. ; Johnson, Mary T. / Effect of hydrogen peroxide on proliferation, apoptosis and interleukin-2 production of Jurkat T cells. In: Biomedical Sciences Instrumentation. 2004 ; Vol. 40. pp. 123-128.
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