Effect of imaging geometry on evaluating natural white-spot lesions using quantitative light-induced fluorescence

Masatoshi Ando, George J. Eckert, George K. Stookey, Domenick T. Zero

Research output: Contribution to journalArticle

13 Scopus citations


The objective of this study was to determine the effect of imaging geometry on evaluating natural white-spot lesions with quantitative light-induced fluorescence (QLF). A total of 34 specimens were prepared from extracted human premolars and permanent molars with white spots on the interproximal surface. The specimens were each adjusted to a final thickness of 3.0 mm. Images were acquired with the QLF system perpendicular to the white spots and at 5° intervals up to 30° above and below the perpendicular. The specimens were rotated around the buccolingual axis of the tooth (pitch angle) and around the long axis of the tooth (roll angle). The averages of fluorescence loss (ΔF, %) and lesion size (mm2) were determined with QLF. Another variable, ΔQ, which was defined as the fluorescence loss integrated over the lesion size (% × mm2), was also calculated. ΔF was smaller when lesions were viewed from the cervical direction (angles less than 90°), and became bigger when viewed from the coronal direction. Roll angle did not significantly affect ΔF. Apparent lesion size diminished with deviations from 90° in both directions for pitch and roll angles. ΔQ was affected by pitch and roll angles with the largest value at 90° and values decreasing in both directions from 90°. In general, there were significant differences for angles larger than 20° from the perpendicular for all three QLF variables. This study suggests that angle is an important factor to control when performing QLF studies; however, small changes (deviations within 20°) have a minimal effect on QLF variables.

Original languageEnglish (US)
Pages (from-to)39-44
Number of pages6
JournalCaries research
Issue number1
StatePublished - Jan 20 2004


  • Angular disparity
  • Angulation
  • Imaging geometry
  • Natural white-spot lesion
  • Pitch angle
  • Quantitative light-induced fluorescence
  • Roll angle

ASJC Scopus subject areas

  • Dentistry(all)

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