Effect of immunisation with cancer procoagulant on the growth of Walker 256 carcinosarcoma cells in rats

M. Kamocka, K. Kaplinska, W. P. Mielicki

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

The effect of preimmunisation with cancer procoagulant (CP) on the growth of Walker 256 carcinosarcoma cells in Wistar Lew/Han/IMP rats in vivo has been analysed. One group of rats was immunised with CP purified from human amnion-chorion membranes. The rest of the animals (control groups) were injected with 0.9% NaCl in Freund's adjuvant or were not immunised at all. When the presence of polyclonal anti-CP antibody was detected in CP-immunised rats' serum, all (CP-immunised and control) animals were injected i.p. with 4.8×105 Walker 256 cells per rat. After 5 days ascitic fluid was collected and viable cells were counted. The complete lack of viable Walker 256 carcinosarcoma cells in 24% of the CP-immunised rats was observed. However, the viable neoplastic cells were present in all control (NaCl-injected and non-immunised) animals. It seems possible that CP plays an important role in tumour progression, so immunisation with CP can reduce the risk of development of malignant disease.

Original languageEnglish (US)
Pages (from-to)119-123
Number of pages5
JournalClinical and Experimental Medicine
Volume6
Issue number3
DOIs
StatePublished - Oct 1 2006
Externally publishedYes

Keywords

  • Cancer growth inhibition
  • Cancer procoagulant
  • Immunisation
  • Walker 256

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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