Effect of Ischemia on Nucleosides and Bases in Rat Liver and Hepatoma 3924A

Konrad Pillwein, Hiremagalur N. Jayaram, George Weber

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

A high-pressure liquid chromatographic method was developed which achieved a separation and quantitation of 20 biologically important nucleosides and bases. The concentrations of pyrimidine nucleosomes and bases, namely deoxycytidine, cytosine, cytidine, uracil, and uridine (22.6, 10.1, 5.2, 2.9, and 2.4 nmol/ml, respectively) were high in plasma, whereas purine nucleosides and bases were present in concentrations less than 2.5 nmol/ml. In erythrocytes, the pools of xanthine, hypoxanthine, and xanthosine were 32-, 27-, and 22-fold larger, respectively, whereas cytidine, uridine, and deoxycytidine were only 21, 12, and 5% of plasma concentrations. The results suggest a compartmental system for transport of some of the purine and pyrimidine nucleosides and bases in the whole blood. Studies on the effect of ischemia on nucleoside and base pools in rat liver indicated marked increases within 30 s in the concentrations of adenine, adenosine, inosine, hypoxanthine, uridine, and xanthine, whereas in hepatoma the effects were less pronounced. By 2 and 5 min ischemia these perturbations were most marked in both liver and hepatoma. These results indicate a need for rapid freeze-clamp preparation of tissue samples to obtain precise and repeatable results in the determination of tissue nucleoside and nucleobase concentrations.

Original languageEnglish (US)
Pages (from-to)3092-3096
Number of pages5
JournalCancer Research
Volume47
Issue number12
StatePublished - Jun 15 1987

Fingerprint

Uridine
Nucleosides
Purine Nucleosides
Hepatocellular Carcinoma
Cytidine
Deoxycytidine
Hypoxanthine
Xanthine
Ischemia
Liver
Pyrimidine Nucleosides
Inosine
Uracil
Nucleosomes
Cytosine
Adenine
Adenosine
Erythrocytes
Pressure

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Pillwein, K., Jayaram, H. N., & Weber, G. (1987). Effect of Ischemia on Nucleosides and Bases in Rat Liver and Hepatoma 3924A. Cancer Research, 47(12), 3092-3096.

Effect of Ischemia on Nucleosides and Bases in Rat Liver and Hepatoma 3924A. / Pillwein, Konrad; Jayaram, Hiremagalur N.; Weber, George.

In: Cancer Research, Vol. 47, No. 12, 15.06.1987, p. 3092-3096.

Research output: Contribution to journalArticle

Pillwein, K, Jayaram, HN & Weber, G 1987, 'Effect of Ischemia on Nucleosides and Bases in Rat Liver and Hepatoma 3924A', Cancer Research, vol. 47, no. 12, pp. 3092-3096.
Pillwein K, Jayaram HN, Weber G. Effect of Ischemia on Nucleosides and Bases in Rat Liver and Hepatoma 3924A. Cancer Research. 1987 Jun 15;47(12):3092-3096.
Pillwein, Konrad ; Jayaram, Hiremagalur N. ; Weber, George. / Effect of Ischemia on Nucleosides and Bases in Rat Liver and Hepatoma 3924A. In: Cancer Research. 1987 ; Vol. 47, No. 12. pp. 3092-3096.
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