Effect of JP-8 jet fuel exposure on protein expression in human keratinocyte cells in culture

F. A. Witzmann, N. A. Monteiro-Riviere, A. O. Inman, M. A. Kimpel, N. M. Pedrick, H. N. Ringham, J. E. Riviere

Research output: Contribution to journalArticle

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Abstract

Dermal exposure to jet fuel is a significant occupational hazard. Previous studies have investigated its absorption and disposition in skin, and the systemic biochemical and immunotoxicological sequelae to exposure. Despite studies of JP-8 jet fuel components in murine, porcine or human keratinocyte cell cultures, proteomic analysis of JP-8 exposure has not been investigated. This study was conducted to examine the effect of JP-8 administration on the human epidermal keratinocyte (HEK) proteome. Using a two-dimensional electrophoretic approach combined with mass spectrometric-based protein identification, we analyzed protein expression in HEK exposed to 0.1% JP-8 in culture medium for 24 h. JP-8 exposure resulted in significant expression differences (p < 0.02) in 35 of the 929 proteins matched and analyzed. Approximately, a third of these alterations were increased in protein expression, two-thirds declined with JP-8 exposure. Peptide mass fingerprint identification of effected proteins revealed a variety of functional implications. In general, altered proteins involved endocytotic/exocytotic mechanisms and their cytoskeletal components, cell stress, and those involved in vesicular function.

Original languageEnglish (US)
Pages (from-to)8-21
Number of pages14
JournalToxicology Letters
Volume160
Issue number1
DOIs
StatePublished - Dec 30 2005

Fingerprint

Keratinocytes
Cell Culture Techniques
Proteins
Skin
Peptide Mapping
Jet fuel
Cellular Structures
Proteome
Cell culture
Proteomics
Culture Media
JP8 aviation fuel
Hazards
Swine
Peptides

Keywords

  • Jet fuel
  • Keratinocyte
  • Mass spectrometry
  • Proteomics
  • Toxicity
  • Two-dimensional electrophoresis

ASJC Scopus subject areas

  • Toxicology

Cite this

Witzmann, F. A., Monteiro-Riviere, N. A., Inman, A. O., Kimpel, M. A., Pedrick, N. M., Ringham, H. N., & Riviere, J. E. (2005). Effect of JP-8 jet fuel exposure on protein expression in human keratinocyte cells in culture. Toxicology Letters, 160(1), 8-21. https://doi.org/10.1016/j.toxlet.2005.06.001

Effect of JP-8 jet fuel exposure on protein expression in human keratinocyte cells in culture. / Witzmann, F. A.; Monteiro-Riviere, N. A.; Inman, A. O.; Kimpel, M. A.; Pedrick, N. M.; Ringham, H. N.; Riviere, J. E.

In: Toxicology Letters, Vol. 160, No. 1, 30.12.2005, p. 8-21.

Research output: Contribution to journalArticle

Witzmann, FA, Monteiro-Riviere, NA, Inman, AO, Kimpel, MA, Pedrick, NM, Ringham, HN & Riviere, JE 2005, 'Effect of JP-8 jet fuel exposure on protein expression in human keratinocyte cells in culture', Toxicology Letters, vol. 160, no. 1, pp. 8-21. https://doi.org/10.1016/j.toxlet.2005.06.001
Witzmann, F. A. ; Monteiro-Riviere, N. A. ; Inman, A. O. ; Kimpel, M. A. ; Pedrick, N. M. ; Ringham, H. N. ; Riviere, J. E. / Effect of JP-8 jet fuel exposure on protein expression in human keratinocyte cells in culture. In: Toxicology Letters. 2005 ; Vol. 160, No. 1. pp. 8-21.
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