Effect of JP-8 jet fuel exposure on protein expression in human keratinocyte cells in culture

F. A. Witzmann, N. A. Monteiro-Riviere, A. O. Inman, M. A. Kimpel, N. M. Pedrick, H. N. Ringham, J. E. Riviere

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

Dermal exposure to jet fuel is a significant occupational hazard. Previous studies have investigated its absorption and disposition in skin, and the systemic biochemical and immunotoxicological sequelae to exposure. Despite studies of JP-8 jet fuel components in murine, porcine or human keratinocyte cell cultures, proteomic analysis of JP-8 exposure has not been investigated. This study was conducted to examine the effect of JP-8 administration on the human epidermal keratinocyte (HEK) proteome. Using a two-dimensional electrophoretic approach combined with mass spectrometric-based protein identification, we analyzed protein expression in HEK exposed to 0.1% JP-8 in culture medium for 24 h. JP-8 exposure resulted in significant expression differences (p < 0.02) in 35 of the 929 proteins matched and analyzed. Approximately, a third of these alterations were increased in protein expression, two-thirds declined with JP-8 exposure. Peptide mass fingerprint identification of effected proteins revealed a variety of functional implications. In general, altered proteins involved endocytotic/exocytotic mechanisms and their cytoskeletal components, cell stress, and those involved in vesicular function.

Original languageEnglish (US)
Pages (from-to)8-21
Number of pages14
JournalToxicology Letters
Volume160
Issue number1
DOIs
StatePublished - Dec 30 2005

Keywords

  • Jet fuel
  • Keratinocyte
  • Mass spectrometry
  • Proteomics
  • Toxicity
  • Two-dimensional electrophoresis

ASJC Scopus subject areas

  • Toxicology

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