Effect of medicaments used in endodontic regeneration technique on the chemical structure of human immature radicular dentin: An in vitro study

Ghaeth H. Yassen, T.M. Gabriel Chu, George Eckert, Jeffrey Platt

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54 Citations (Scopus)

Abstract

Introduction: The aim of this study was to investigate the effect of 3 intracanal medicaments used in pulp regeneration on the chemical structure of radicular dentin. Methods: Human immature radicular dentin specimens were exposed to triple antibiotic paste (tripaste), double antibiotic paste (bipaste), calcium hydroxide [(Ca(OH)2] paste, or deionized water (control) for 1, 2, or 4 weeks. After each time point, specimens were examined with attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy to analyze the relative loss of organic and inorganic components by using phosphate/amide I ratios. Data were analyzed by using analysis of variance followed by post hoc comparisons. Results: Phosphate/amide ratios were significantly different between the 4 groups at all time points (P <.0001): Ca(OH)2-treated dentin > untreated control dentin > bipaste-treated dentin > tripaste-treated dentin. For bipaste groups, 4-week treated dentin had significantly lower phosphate/amide I ratios than 1- and 2-week treated dentin (P <.05); however, phosphate/amide I ratios of 1- and 2-week treated dentin did not have a significant difference. For tripaste groups, 4-week treated dentin had a significantly higher phosphate/amide I ratio than 1- and 2-week treated dentin (P <.001), and phosphate/amide I ratio of 1-week treated dentin was significantly higher than that of 2-week treated dentin (P =.04). No significant time effect for Ca(OH)2-treated dentin or untreated control dentin was found (P >.05). Conclusions: The results suggested a superficial collagen degradation or demineralization of radicular dentin caused by Ca(OH)2 or antibiotic pastes, respectively, after 1, 2, or 4 weeks of exposure.

Original languageEnglish
Pages (from-to)269-273
Number of pages5
JournalJournal of Endodontics
Volume39
Issue number2
DOIs
StatePublished - Feb 2013

Fingerprint

Endodontics
Dentin
Regeneration
Ointments
Amides
Anti-Bacterial Agents
Phosphates
Calcium Hydroxide
In Vitro Techniques
Fourier Transform Infrared Spectroscopy
Analysis of Variance
Collagen
Water

Keywords

  • Calcium hydroxide
  • double antibiotic paste
  • endodontic regeneration
  • Fourier transform infrared spectroscopy
  • triple antibiotic paste

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

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title = "Effect of medicaments used in endodontic regeneration technique on the chemical structure of human immature radicular dentin: An in vitro study",
abstract = "Introduction: The aim of this study was to investigate the effect of 3 intracanal medicaments used in pulp regeneration on the chemical structure of radicular dentin. Methods: Human immature radicular dentin specimens were exposed to triple antibiotic paste (tripaste), double antibiotic paste (bipaste), calcium hydroxide [(Ca(OH)2] paste, or deionized water (control) for 1, 2, or 4 weeks. After each time point, specimens were examined with attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy to analyze the relative loss of organic and inorganic components by using phosphate/amide I ratios. Data were analyzed by using analysis of variance followed by post hoc comparisons. Results: Phosphate/amide ratios were significantly different between the 4 groups at all time points (P <.0001): Ca(OH)2-treated dentin > untreated control dentin > bipaste-treated dentin > tripaste-treated dentin. For bipaste groups, 4-week treated dentin had significantly lower phosphate/amide I ratios than 1- and 2-week treated dentin (P <.05); however, phosphate/amide I ratios of 1- and 2-week treated dentin did not have a significant difference. For tripaste groups, 4-week treated dentin had a significantly higher phosphate/amide I ratio than 1- and 2-week treated dentin (P <.001), and phosphate/amide I ratio of 1-week treated dentin was significantly higher than that of 2-week treated dentin (P =.04). No significant time effect for Ca(OH)2-treated dentin or untreated control dentin was found (P >.05). Conclusions: The results suggested a superficial collagen degradation or demineralization of radicular dentin caused by Ca(OH)2 or antibiotic pastes, respectively, after 1, 2, or 4 weeks of exposure.",
keywords = "Calcium hydroxide, double antibiotic paste, endodontic regeneration, Fourier transform infrared spectroscopy, triple antibiotic paste",
author = "Yassen, {Ghaeth H.} and Chu, {T.M. Gabriel} and George Eckert and Jeffrey Platt",
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T1 - Effect of medicaments used in endodontic regeneration technique on the chemical structure of human immature radicular dentin

T2 - An in vitro study

AU - Yassen, Ghaeth H.

AU - Chu, T.M. Gabriel

AU - Eckert, George

AU - Platt, Jeffrey

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N2 - Introduction: The aim of this study was to investigate the effect of 3 intracanal medicaments used in pulp regeneration on the chemical structure of radicular dentin. Methods: Human immature radicular dentin specimens were exposed to triple antibiotic paste (tripaste), double antibiotic paste (bipaste), calcium hydroxide [(Ca(OH)2] paste, or deionized water (control) for 1, 2, or 4 weeks. After each time point, specimens were examined with attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy to analyze the relative loss of organic and inorganic components by using phosphate/amide I ratios. Data were analyzed by using analysis of variance followed by post hoc comparisons. Results: Phosphate/amide ratios were significantly different between the 4 groups at all time points (P <.0001): Ca(OH)2-treated dentin > untreated control dentin > bipaste-treated dentin > tripaste-treated dentin. For bipaste groups, 4-week treated dentin had significantly lower phosphate/amide I ratios than 1- and 2-week treated dentin (P <.05); however, phosphate/amide I ratios of 1- and 2-week treated dentin did not have a significant difference. For tripaste groups, 4-week treated dentin had a significantly higher phosphate/amide I ratio than 1- and 2-week treated dentin (P <.001), and phosphate/amide I ratio of 1-week treated dentin was significantly higher than that of 2-week treated dentin (P =.04). No significant time effect for Ca(OH)2-treated dentin or untreated control dentin was found (P >.05). Conclusions: The results suggested a superficial collagen degradation or demineralization of radicular dentin caused by Ca(OH)2 or antibiotic pastes, respectively, after 1, 2, or 4 weeks of exposure.

AB - Introduction: The aim of this study was to investigate the effect of 3 intracanal medicaments used in pulp regeneration on the chemical structure of radicular dentin. Methods: Human immature radicular dentin specimens were exposed to triple antibiotic paste (tripaste), double antibiotic paste (bipaste), calcium hydroxide [(Ca(OH)2] paste, or deionized water (control) for 1, 2, or 4 weeks. After each time point, specimens were examined with attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy to analyze the relative loss of organic and inorganic components by using phosphate/amide I ratios. Data were analyzed by using analysis of variance followed by post hoc comparisons. Results: Phosphate/amide ratios were significantly different between the 4 groups at all time points (P <.0001): Ca(OH)2-treated dentin > untreated control dentin > bipaste-treated dentin > tripaste-treated dentin. For bipaste groups, 4-week treated dentin had significantly lower phosphate/amide I ratios than 1- and 2-week treated dentin (P <.05); however, phosphate/amide I ratios of 1- and 2-week treated dentin did not have a significant difference. For tripaste groups, 4-week treated dentin had a significantly higher phosphate/amide I ratio than 1- and 2-week treated dentin (P <.001), and phosphate/amide I ratio of 1-week treated dentin was significantly higher than that of 2-week treated dentin (P =.04). No significant time effect for Ca(OH)2-treated dentin or untreated control dentin was found (P >.05). Conclusions: The results suggested a superficial collagen degradation or demineralization of radicular dentin caused by Ca(OH)2 or antibiotic pastes, respectively, after 1, 2, or 4 weeks of exposure.

KW - Calcium hydroxide

KW - double antibiotic paste

KW - endodontic regeneration

KW - Fourier transform infrared spectroscopy

KW - triple antibiotic paste

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