Effect of nicotine on cariogenic virulence of Streptococcus mutans

Mingyun Li, Ruijie Huang, Xuedong Zhou, Wei Qiu, Xin Xu, Richard Gregory

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Nicotine has well-documented effects on the growth and colonization of Streptococcus mutans. This study attempts to investigate the effects of nicotine on pathogenic factors of S. mutans, such as the effect on biofilm formation and viability, expression of pathogenic genes, and metabolites of S. mutans. The results demonstrated that addition of nicotine did not significantly influence the viability of S. mutans cells. The biofilms became increasingly compact as the concentrations of nicotine increased. The expression of virulence genes, such as ldh and phosphotransferase system (PTS)-associated genes, was upregulated, and nlmC was upregulated significantly, while ftf was downregulated. The lactate concentration of S. mutans grown in 1 mg/mL of nicotine was increased up to twofold over either biofilm or planktonic cells grown without nicotine. Changes in the metabolites involved in central carbon metabolism from sucrose indicated that most selected metabolites were detectable and influenced by increased concentrations of nicotine. This study demonstrated that nicotine can influence the pathogenicity of S. mutans and may lead to increased dental caries through the production of more lactate and the upregulation of virulence genes.

Original languageEnglish (US)
Pages (from-to)1-8
Number of pages8
JournalFolia Microbiologica
DOIs
StateAccepted/In press - Jul 6 2016

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Streptococcus mutans
Nicotine
Virulence
Biofilms
Lactic Acid
Gene Expression
Dental Caries
Genes
Sucrose
Phosphotransferases
Up-Regulation
Carbon
Down-Regulation
Growth

ASJC Scopus subject areas

  • Microbiology

Cite this

Effect of nicotine on cariogenic virulence of Streptococcus mutans. / Li, Mingyun; Huang, Ruijie; Zhou, Xuedong; Qiu, Wei; Xu, Xin; Gregory, Richard.

In: Folia Microbiologica, 06.07.2016, p. 1-8.

Research output: Contribution to journalArticle

Li, Mingyun ; Huang, Ruijie ; Zhou, Xuedong ; Qiu, Wei ; Xu, Xin ; Gregory, Richard. / Effect of nicotine on cariogenic virulence of Streptococcus mutans. In: Folia Microbiologica. 2016 ; pp. 1-8.
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