Effect of nicotine on dual-species biofilms of Streptococcus mutans and Streptococcus sanguinis

Mingyun Li, Ruijie Huang, Xuedong Zhou, Keke Zhang, Xin Zheng, Richard Gregory

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Both Streptococcus mutans and Streptococcus sanguinis are normal bacterial inhabitants of dental plaque. Streptococcus mutans is the major agent causing dental caries. It has been well documented that nicotine affects the growth of S. mutans. This study investigated the effect of nicotine on mono- and dual-species growth of S. mutans and S. sanguinis. The results indicate that nicotine has no significant effect on S. sanguinis grown in either mono- or dual-species biofilms. However, nicotine significantly increased (P < 0.05) the growth of S. mutans in dual-species biofilm formation. In addition, the CFU level of S. sanguinis was higher than S. mutans without nicotine in the culture. With the addition of nicotine, the level of S. mutans biofilm was significantly enhanced as the nicotine concentration increased over the level of S. sanguinis in dual-species biofilm, and we also got the same result from the fluorescence in situ hybridization detecting the two bacteria grown in biofilm formation. The exopolysaccharide (EPS) of S. mutans has also been increased by the increasing nicotine concentration, while the EPS of S. sanguinis was decreased or inhibited by the affected nicotine. The data further confirm that nicotine is able to enhance the growth of S. mutans.

Original languageEnglish
Pages (from-to)125-132
Number of pages8
JournalFEMS Microbiology Letters
Volume350
Issue number2
DOIs
StatePublished - Jan 2014

Fingerprint

Streptococcus mutans
Biofilms
Streptococcus
Nicotine
Growth
Dental Plaque
Dental Caries
Fluorescence In Situ Hybridization
Bacteria

Keywords

  • Exopolysaccharide
  • Fluorescence in situ hybridization
  • Nicotine
  • Streptococcus mutans
  • Streptococcus sanguinis

ASJC Scopus subject areas

  • Microbiology
  • Genetics
  • Molecular Biology

Cite this

Effect of nicotine on dual-species biofilms of Streptococcus mutans and Streptococcus sanguinis. / Li, Mingyun; Huang, Ruijie; Zhou, Xuedong; Zhang, Keke; Zheng, Xin; Gregory, Richard.

In: FEMS Microbiology Letters, Vol. 350, No. 2, 01.2014, p. 125-132.

Research output: Contribution to journalArticle

Li, Mingyun ; Huang, Ruijie ; Zhou, Xuedong ; Zhang, Keke ; Zheng, Xin ; Gregory, Richard. / Effect of nicotine on dual-species biofilms of Streptococcus mutans and Streptococcus sanguinis. In: FEMS Microbiology Letters. 2014 ; Vol. 350, No. 2. pp. 125-132.
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