Effect of nicotine on growth and metabolism of Streptococcus mutans

Ruijie Huang, Mingyun Li, Richard Gregory

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

Streptococcus mutans is a key contributor to dental caries. Smokers have a higher number of caries-affected teeth than do nonsmokers, but the association among tobacco, nicotine, caries, and S. mutans growth has not been investigated in detail. Seven S. mutans strains - UA159, UA130, 10449, A32-2, NG8, LM7, and OMZ175 - were used in the present study. The minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), minimum biofilm inhibitory concentration (MBIC), planktonic cell growth, biofilm formation, metabolism, and structure (determined using scanning electron microscopy) of the seven strains treated with different concentrations of nicotine (0-32 mg ml-1) were investigated. The MIC, MBC, and MBIC were 16 mg ml-1 (0.1 M), 32 mg ml-1 (0.2 M), and 16 mg ml-1 (0.1 M), respectively, for most of the S. mutans strains. Growth of planktonic S. mutans cells was significantly repressed by 2.0-8.0 mg ml-1 of nicotine. Biofilm formation and metabolic activity of S. mutans was increased in a nicotine-dependent manner up to 16.0 mg ml-1 of nicotine. Scanning electron microscopy revealed that S. mutans treated with a high concentration of nicotine a had thicker biofilm and more spherical bacterial cells. In summary, nicotine enhances S. mutans biofilm formation and biofilm metabolic activity. These results suggest that smoking can increase the development of caries by fostering increased formation of S. mutans biofilm on tooth surfaces.

Original languageEnglish
Pages (from-to)319-325
Number of pages7
JournalEuropean Journal of Oral Sciences
Volume120
Issue number4
DOIs
StatePublished - Aug 2012

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Streptococcus mutans
Nicotine
Biofilms
Growth
Microbial Sensitivity Tests
Electron Scanning Microscopy
Tooth
Foster Home Care
Dental Caries
Tobacco
Smoking

Keywords

  • Biofilm
  • Dental caries
  • Tobacco
  • XTT

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Effect of nicotine on growth and metabolism of Streptococcus mutans. / Huang, Ruijie; Li, Mingyun; Gregory, Richard.

In: European Journal of Oral Sciences, Vol. 120, No. 4, 08.2012, p. 319-325.

Research output: Contribution to journalArticle

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