Effect of prenatal alcohol exposure on bony craniofacial development: A mouse MicroCT study

Li Shen, Huisi Ai, Yun Liang, Xiaowei Ren, Charles Bruce Anthony, Charles R. Goodlett, Richard Ward, Feng Zhou

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Craniofacial bone dysmorphology is an important but under-explored potential diagnostic feature of fetal alcohol spectrum disorders. This study used longitudinal MicroCT 3D imaging to examine the effect of prenatal alcohol exposure on craniofacial bone growth in a mouse model. C57BL/6J dams were divided into 3 groups: alcohol 4.2% v/v in PMI® liquid diet (ALC), 2 weeks prior to and during pregnancy from embryonic (E) days 7-E16; pair-fed controls (PF), isocalorically matched to the ALC group; chow controls (CHOW), given ad libitum chow and water. The MicroCT scans were performed on pups on postnatal days 7 (P7) and P21. The volumes of the neurocranium (volume encased by the frontal, parietal, and occipital bones) and the viscerocranium (volume encased by the mandible and nasal bone), along with total skull bone volume, head size, and head circumference were evaluated using general linear models and discriminant analyses. The pups in the alcohol-treated group, when compared to the chow-fed controls (ALC vs CHOW) and the isocaloric-fed controls (ALC vs PF), showed differences in head size and circumference at P7 and P21, the total skull volume and parietal bone volume at P7, and volume of all the tested bones except nasal at P21. There was a growth trend of ALC<CHOW and ALC<PF. While covarying for gender and head size or circumference, the treatment affected the total skull and mandible at P7 (ALC>CHOW), and the total skull, parietal bone, and occipital bone at P21 (ALC<CHOW, ALC<PF). While covarying for the P7 measures, the treatment affected only the 3 neurocranial bones at P21 (ALC<CHOW, ALC<PF). Discriminant analysis sensitively selected between ALC and CHOW (AUC=0.967), between ALC and PF (AUC=0.995), and between PF and CHOW (AUC=0.805). These results supported our hypothesis that craniofacial bones might be a reliable and sensitive indicator for the diagnosis of prenatal alcohol exposure. Significantly, we found that the neurocranium (upper skull) was more sensitive to alcohol than the viscerocranium (face).

Original languageEnglish
Pages (from-to)405-415
Number of pages11
JournalAlcohol
Volume47
Issue number5
DOIs
StatePublished - Aug 2013

Fingerprint

X-Ray Microtomography
Parietal Bone
Bone
Skull
alcohol
Alcohols
Bone and Bones
Occipital Bone
Nasal Bone
Area Under Curve
Head
Discriminant Analysis
Frontal Bone
Fetal Alcohol Spectrum Disorders
Bone Development
Prenatal Diagnosis
Mandible
Longitudinal Studies
Linear Models
Diet

Keywords

  • Craniofacial bone
  • Diagnosis
  • Facial dysmorphology
  • Prenatal alcohol

ASJC Scopus subject areas

  • Biochemistry
  • Medicine(all)
  • Behavioral Neuroscience
  • Neurology
  • Toxicology
  • Health(social science)

Cite this

Effect of prenatal alcohol exposure on bony craniofacial development : A mouse MicroCT study. / Shen, Li; Ai, Huisi; Liang, Yun; Ren, Xiaowei; Anthony, Charles Bruce; Goodlett, Charles R.; Ward, Richard; Zhou, Feng.

In: Alcohol, Vol. 47, No. 5, 08.2013, p. 405-415.

Research output: Contribution to journalArticle

Shen, Li ; Ai, Huisi ; Liang, Yun ; Ren, Xiaowei ; Anthony, Charles Bruce ; Goodlett, Charles R. ; Ward, Richard ; Zhou, Feng. / Effect of prenatal alcohol exposure on bony craniofacial development : A mouse MicroCT study. In: Alcohol. 2013 ; Vol. 47, No. 5. pp. 405-415.
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