Effect of prolonged sitting and breaks in sitting time on endothelial function

Saurabh S. Thosar, Sylvanna L. Bielko, Kieren J. Mather, Jeanne D. Johnston, Janet P. Wallace

Research output: Contribution to journalArticle

82 Citations (Scopus)

Abstract

Sitting time (ST) is associated with cardiovascular disease risk factors, whereas breaking ST has been reported to be beneficial for reducing cardiovascular risk. Purpose: The objective of this study is to examine the effects of breaking ST on superficial femoral artery (SFA) endothelial function. Hypotheses: 1) Prolonged sitting would induce endothelial dysfunction and changes in shear forces, and 2) breaking ST with brief periods of activity would prevent attenuation in endothelial function. Methods: Twelve nonobese men (24.2 T 4.2 yr) participated in two randomized 3-h sitting trials. In the sitting (SIT) trial, subjects were seated on a firmly cushioned chair for 3 h without moving their lower extremities. In the breaking ST trial (ACT), subjects sat similar to the SIT trial but walked on a treadmill for 5 min at 2 mph at 30 min, 1 h 30 min, and 2 h 30 min during the sitting interval. SFA flow-mediated dilation (FMD) was assessed at baseline, 1 h, 2 h, and 3 h in each trial. Statistical analyses were performed using dependent variables SFA FMD and shear rates. Significance was set at P ≤ 0.05. Results: In the SIT trial, there was a significant decline in SFA FMD from baseline to 3 h (baseline, 4.72% × 3.78%; 1 h, 0.52% × 0.85%; 2 h, 1.66% × 1.11%; 3 h, 2.2% × 2.15; P < 0.05 by ANOVA) accompanied by a decline in mean shear rate and antegrade shear rate but no difference in shear rate (area under the curve). By two-way repeatedmeasures ANOVA, ACT prevented the sitting-induced decline in FMD (baseline, 4.5% × 2.3%; 1 h, 5.04% × 2.85%; 2 h, 5.28% × 5.05%; 3 h, 6.9% × 4.5%) along with no decline in shear rates. Conclusion: Three hours of sitting resulted in a significant impairment in shear rate and SFA FMD. When light activity breaks were introduced hourly during sitting, the decline in FMD was prevented.

Original languageEnglish (US)
Pages (from-to)843-849
Number of pages7
JournalMedicine and Science in Sports and Exercise
Volume47
Issue number4
DOIs
StatePublished - Apr 25 2015

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Femoral Artery
Dilatation
Analysis of Variance
Area Under Curve
Lower Extremity
Cardiovascular Diseases
Light

Keywords

  • flow-mediated dilation
  • intermittent activity
  • low shear rate
  • sedentary activity

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine
  • Physical Therapy, Sports Therapy and Rehabilitation

Cite this

Effect of prolonged sitting and breaks in sitting time on endothelial function. / Thosar, Saurabh S.; Bielko, Sylvanna L.; Mather, Kieren J.; Johnston, Jeanne D.; Wallace, Janet P.

In: Medicine and Science in Sports and Exercise, Vol. 47, No. 4, 25.04.2015, p. 843-849.

Research output: Contribution to journalArticle

Thosar, Saurabh S. ; Bielko, Sylvanna L. ; Mather, Kieren J. ; Johnston, Jeanne D. ; Wallace, Janet P. / Effect of prolonged sitting and breaks in sitting time on endothelial function. In: Medicine and Science in Sports and Exercise. 2015 ; Vol. 47, No. 4. pp. 843-849.
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abstract = "Sitting time (ST) is associated with cardiovascular disease risk factors, whereas breaking ST has been reported to be beneficial for reducing cardiovascular risk. Purpose: The objective of this study is to examine the effects of breaking ST on superficial femoral artery (SFA) endothelial function. Hypotheses: 1) Prolonged sitting would induce endothelial dysfunction and changes in shear forces, and 2) breaking ST with brief periods of activity would prevent attenuation in endothelial function. Methods: Twelve nonobese men (24.2 T 4.2 yr) participated in two randomized 3-h sitting trials. In the sitting (SIT) trial, subjects were seated on a firmly cushioned chair for 3 h without moving their lower extremities. In the breaking ST trial (ACT), subjects sat similar to the SIT trial but walked on a treadmill for 5 min at 2 mph at 30 min, 1 h 30 min, and 2 h 30 min during the sitting interval. SFA flow-mediated dilation (FMD) was assessed at baseline, 1 h, 2 h, and 3 h in each trial. Statistical analyses were performed using dependent variables SFA FMD and shear rates. Significance was set at P ≤ 0.05. Results: In the SIT trial, there was a significant decline in SFA FMD from baseline to 3 h (baseline, 4.72{\%} × 3.78{\%}; 1 h, 0.52{\%} × 0.85{\%}; 2 h, 1.66{\%} × 1.11{\%}; 3 h, 2.2{\%} × 2.15; P < 0.05 by ANOVA) accompanied by a decline in mean shear rate and antegrade shear rate but no difference in shear rate (area under the curve). By two-way repeatedmeasures ANOVA, ACT prevented the sitting-induced decline in FMD (baseline, 4.5{\%} × 2.3{\%}; 1 h, 5.04{\%} × 2.85{\%}; 2 h, 5.28{\%} × 5.05{\%}; 3 h, 6.9{\%} × 4.5{\%}) along with no decline in shear rates. Conclusion: Three hours of sitting resulted in a significant impairment in shear rate and SFA FMD. When light activity breaks were introduced hourly during sitting, the decline in FMD was prevented.",
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N2 - Sitting time (ST) is associated with cardiovascular disease risk factors, whereas breaking ST has been reported to be beneficial for reducing cardiovascular risk. Purpose: The objective of this study is to examine the effects of breaking ST on superficial femoral artery (SFA) endothelial function. Hypotheses: 1) Prolonged sitting would induce endothelial dysfunction and changes in shear forces, and 2) breaking ST with brief periods of activity would prevent attenuation in endothelial function. Methods: Twelve nonobese men (24.2 T 4.2 yr) participated in two randomized 3-h sitting trials. In the sitting (SIT) trial, subjects were seated on a firmly cushioned chair for 3 h without moving their lower extremities. In the breaking ST trial (ACT), subjects sat similar to the SIT trial but walked on a treadmill for 5 min at 2 mph at 30 min, 1 h 30 min, and 2 h 30 min during the sitting interval. SFA flow-mediated dilation (FMD) was assessed at baseline, 1 h, 2 h, and 3 h in each trial. Statistical analyses were performed using dependent variables SFA FMD and shear rates. Significance was set at P ≤ 0.05. Results: In the SIT trial, there was a significant decline in SFA FMD from baseline to 3 h (baseline, 4.72% × 3.78%; 1 h, 0.52% × 0.85%; 2 h, 1.66% × 1.11%; 3 h, 2.2% × 2.15; P < 0.05 by ANOVA) accompanied by a decline in mean shear rate and antegrade shear rate but no difference in shear rate (area under the curve). By two-way repeatedmeasures ANOVA, ACT prevented the sitting-induced decline in FMD (baseline, 4.5% × 2.3%; 1 h, 5.04% × 2.85%; 2 h, 5.28% × 5.05%; 3 h, 6.9% × 4.5%) along with no decline in shear rates. Conclusion: Three hours of sitting resulted in a significant impairment in shear rate and SFA FMD. When light activity breaks were introduced hourly during sitting, the decline in FMD was prevented.

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