Effect of split low dose total body irradiation on SFFV mRNA, genomic DNA and protein expression in mice infected with the friend virus complex

Rong Nian Shen, Li Lu, Maureen Harrington, Carolyn Srivastava, Young June Kim, Shang Zhen Zhou, Bo Wu, Sandra Ruscetti, Hal Broxmeyer

Research output: Contribution to journalArticle

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Abstract

DBA/2 mice infected with lethal dosages of Friend virus complex (FVC) can be 100% cured by split-dose total body irradiation (TBI) at 150 cGy, an effect associated with the restoration of the cellular immunity which is compromised by the virus. The exact mechanism underlying the curative effect is unknown, but it may involve the interferon (IFN) system and interleukin-2 (IL-2) production. Initial studies indicated that TBI did not directly inactivate the virus, suggesting that irradiation either acted on the target cells for virus replication or on other cells mediating the effect. We have now examined the effect of this relatively low dose TBI on replication, transcription, and protein expression of the Friend virus. Northern blot analysis revealed that in FVC infected mice treated with curative low dose TBI, no spleen focus-forming virus (SFFV)-specific mRNA species were detected. Southern blot analysis revealed that a 6.0 kb SFFV fragment could be detected in infected, untreated spleen cells, but not in cells from FVC-infected mice treated with TBI, or in uninfected spleen cells. Western blot analysis revealed that the SFFV envelope glycoprotein was expressed in the spleen cells from untreated FVC infected mice, but not in the cells from TBI treated FVC infected mice. These results, consistent with our previous findings of greatly reduced spleen focus forming units in mice with FVC which had been treated with this regimen of TBI, suggest the possibility of using such treatments in other retroviral associated disorders.

Original languageEnglish
Pages (from-to)225-229
Number of pages5
JournalLeukemia
Volume5
Issue number3
StatePublished - Mar 1991

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Spleen Focus-Forming Viruses
Friend murine leukemia virus
Whole-Body Irradiation
Messenger RNA
DNA
Spleen
Proteins
Viruses
Inbred DBA Mouse
Virus Replication
Southern Blotting
Cellular Immunity
Northern Blotting
Interferons
Interleukin-2
Glycoproteins
Western Blotting

ASJC Scopus subject areas

  • Cancer Research
  • Hematology

Cite this

Effect of split low dose total body irradiation on SFFV mRNA, genomic DNA and protein expression in mice infected with the friend virus complex. / Shen, Rong Nian; Lu, Li; Harrington, Maureen; Srivastava, Carolyn; Kim, Young June; Zhou, Shang Zhen; Wu, Bo; Ruscetti, Sandra; Broxmeyer, Hal.

In: Leukemia, Vol. 5, No. 3, 03.1991, p. 225-229.

Research output: Contribution to journalArticle

Shen, Rong Nian ; Lu, Li ; Harrington, Maureen ; Srivastava, Carolyn ; Kim, Young June ; Zhou, Shang Zhen ; Wu, Bo ; Ruscetti, Sandra ; Broxmeyer, Hal. / Effect of split low dose total body irradiation on SFFV mRNA, genomic DNA and protein expression in mice infected with the friend virus complex. In: Leukemia. 1991 ; Vol. 5, No. 3. pp. 225-229.
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