Effect of streptozotocin-induced diabetes mellitus on release of vasoactive intestinal polypeptide from rodent small intestine

Thomas Nowak, William W. Chey, Ta Min Chang, John P. Weisbruch, Gary Fouquet

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Representative longitudinal muscle strips (6 × 10 mm) from proximal and distal small intestine were excised from control and streptozotocin-treated rats after one month of untreated and insulin-treated diabetes. Untreated diabetes significantly reduced tissue concentrations of vasoactive intestinal polypeptide (VIP) at both intestinal loci. Insulin treatment of the diabetic animals restored tissue VIP concentrations to control group levels, although the beneficial effect of insulin treatment was only significant in the duodenum. Spontaneous release of VIP was significantly attenuated by untreated diabetes at both intestinal sites. In the duodenum, insulin treatment of the diabetic animals restored VIP release to levels indistinguishable from control group values. In the ileum, insulin treatment produced levels of VIP release that were not significantly different from those of the control and untreated diabetic groups. Tetrodotoxin (5 × 10-6 M) significantly-but incompletely-inhibited VIP release from control group animals at both intestinal sites. These observations indicate that diabetes mellitus significantly diminishes VIP tissue concentrations and release from intestinal myenteric nerves. These abnormalities improve with insulin treatment. However, the mechanisms of VIP release from proximal and distal intestine appear to differ not only in their response to the diabetic state, but also in their response to insulin treatment.

Original languageEnglish
Pages (from-to)828-836
Number of pages9
JournalDigestive Diseases and Sciences
Volume40
Issue number4
DOIs
StatePublished - Apr 1995

Fingerprint

Experimental Diabetes Mellitus
Vasoactive Intestinal Peptide
Small Intestine
Rodentia
Diabetes Mellitus
Insulin
Duodenum
Control Groups
Tetrodotoxin
Streptozocin
Ileum
Intestines
Muscles

Keywords

  • diabetes mellitus
  • gastrointestinal motility
  • vasoactive intestinal polypeptide

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Effect of streptozotocin-induced diabetes mellitus on release of vasoactive intestinal polypeptide from rodent small intestine. / Nowak, Thomas; Chey, William W.; Chang, Ta Min; Weisbruch, John P.; Fouquet, Gary.

In: Digestive Diseases and Sciences, Vol. 40, No. 4, 04.1995, p. 828-836.

Research output: Contribution to journalArticle

Nowak, Thomas ; Chey, William W. ; Chang, Ta Min ; Weisbruch, John P. ; Fouquet, Gary. / Effect of streptozotocin-induced diabetes mellitus on release of vasoactive intestinal polypeptide from rodent small intestine. In: Digestive Diseases and Sciences. 1995 ; Vol. 40, No. 4. pp. 828-836.
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