Effect of substance P on opossum duodenal smooth muscle

Thomas Nowak, Sinn Anuras

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Opossum duodenum was cut into strips measuring 2.0×15.0 mm. Strips cut along the oral-caudal axis were called longitudinal strips, while those cut 90° to that axis were called circular strips. Each strip was placed in a heated, oxygenated organ bath and attached to a force-displacement transducer. Substance P produced tonic contraction in longitudinal strips and tonic and phasic contraction in circular strips. The ED50 for longitudinal and circular muscle was 1.9×10-7 M and 2.8×10-7 M, respectively. Longitudinal muscle was 1.3 times more sensitive to substance P than circular muscle. Phenoxybenzamine, atropine, curare, propanolol, haloperidol, and tetrodotoxin had no effect on the substance P-produced contractions in circular and longitudinal muscle. Trifluoperazine (10-5 and 10-4 M), D600 (10-7 M), and nifedipine (10-8 and 10-7) inhibited both tonic and phasic contraction in circular and longitudinal strips. These studies suggest that substance P acts on both muscle layers at a site located at the muscle cell and that it produces tonic and phasic contraction through similar calcium-activating pathways.

Original languageEnglish (US)
Pages (from-to)664-668
Number of pages5
JournalDigestive Diseases and Sciences
Volume30
Issue number7
DOIs
StatePublished - Jul 1985
Externally publishedYes

Fingerprint

Opossums
Substance P
Smooth Muscle
Muscles
Gallopamil
Curare
Trifluoperazine
Phenoxybenzamine
Tetrodotoxin
Haloperidol
Nifedipine
Transducers
Baths
Atropine
Duodenum
Propranolol
Muscle Cells
Calcium

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Effect of substance P on opossum duodenal smooth muscle. / Nowak, Thomas; Anuras, Sinn.

In: Digestive Diseases and Sciences, Vol. 30, No. 7, 07.1985, p. 664-668.

Research output: Contribution to journalArticle

@article{5a66e3009a614e0f830956b86f4b0322,
title = "Effect of substance P on opossum duodenal smooth muscle",
abstract = "Opossum duodenum was cut into strips measuring 2.0×15.0 mm. Strips cut along the oral-caudal axis were called longitudinal strips, while those cut 90° to that axis were called circular strips. Each strip was placed in a heated, oxygenated organ bath and attached to a force-displacement transducer. Substance P produced tonic contraction in longitudinal strips and tonic and phasic contraction in circular strips. The ED50 for longitudinal and circular muscle was 1.9×10-7 M and 2.8×10-7 M, respectively. Longitudinal muscle was 1.3 times more sensitive to substance P than circular muscle. Phenoxybenzamine, atropine, curare, propanolol, haloperidol, and tetrodotoxin had no effect on the substance P-produced contractions in circular and longitudinal muscle. Trifluoperazine (10-5 and 10-4 M), D600 (10-7 M), and nifedipine (10-8 and 10-7) inhibited both tonic and phasic contraction in circular and longitudinal strips. These studies suggest that substance P acts on both muscle layers at a site located at the muscle cell and that it produces tonic and phasic contraction through similar calcium-activating pathways.",
author = "Thomas Nowak and Sinn Anuras",
year = "1985",
month = "7",
doi = "10.1007/BF01308416",
language = "English (US)",
volume = "30",
pages = "664--668",
journal = "Digestive Diseases and Sciences",
issn = "0163-2116",
publisher = "Springer New York",
number = "7",

}

TY - JOUR

T1 - Effect of substance P on opossum duodenal smooth muscle

AU - Nowak, Thomas

AU - Anuras, Sinn

PY - 1985/7

Y1 - 1985/7

N2 - Opossum duodenum was cut into strips measuring 2.0×15.0 mm. Strips cut along the oral-caudal axis were called longitudinal strips, while those cut 90° to that axis were called circular strips. Each strip was placed in a heated, oxygenated organ bath and attached to a force-displacement transducer. Substance P produced tonic contraction in longitudinal strips and tonic and phasic contraction in circular strips. The ED50 for longitudinal and circular muscle was 1.9×10-7 M and 2.8×10-7 M, respectively. Longitudinal muscle was 1.3 times more sensitive to substance P than circular muscle. Phenoxybenzamine, atropine, curare, propanolol, haloperidol, and tetrodotoxin had no effect on the substance P-produced contractions in circular and longitudinal muscle. Trifluoperazine (10-5 and 10-4 M), D600 (10-7 M), and nifedipine (10-8 and 10-7) inhibited both tonic and phasic contraction in circular and longitudinal strips. These studies suggest that substance P acts on both muscle layers at a site located at the muscle cell and that it produces tonic and phasic contraction through similar calcium-activating pathways.

AB - Opossum duodenum was cut into strips measuring 2.0×15.0 mm. Strips cut along the oral-caudal axis were called longitudinal strips, while those cut 90° to that axis were called circular strips. Each strip was placed in a heated, oxygenated organ bath and attached to a force-displacement transducer. Substance P produced tonic contraction in longitudinal strips and tonic and phasic contraction in circular strips. The ED50 for longitudinal and circular muscle was 1.9×10-7 M and 2.8×10-7 M, respectively. Longitudinal muscle was 1.3 times more sensitive to substance P than circular muscle. Phenoxybenzamine, atropine, curare, propanolol, haloperidol, and tetrodotoxin had no effect on the substance P-produced contractions in circular and longitudinal muscle. Trifluoperazine (10-5 and 10-4 M), D600 (10-7 M), and nifedipine (10-8 and 10-7) inhibited both tonic and phasic contraction in circular and longitudinal strips. These studies suggest that substance P acts on both muscle layers at a site located at the muscle cell and that it produces tonic and phasic contraction through similar calcium-activating pathways.

UR - http://www.scopus.com/inward/record.url?scp=0021810044&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021810044&partnerID=8YFLogxK

U2 - 10.1007/BF01308416

DO - 10.1007/BF01308416

M3 - Article

C2 - 2408832

AN - SCOPUS:0021810044

VL - 30

SP - 664

EP - 668

JO - Digestive Diseases and Sciences

JF - Digestive Diseases and Sciences

SN - 0163-2116

IS - 7

ER -