Effect of the phosphodiesterase-5 inhibitor zaprinast on ischemia-reperfusion injury in rats

Anthony J. Polcari, Ahmer V. Farooq, Michael E. Woods, Matthew S. Ripsch, Maria Picken, Thomas M.T. Turk, Fletcher A. White

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Abstract

Purpose: The cardiac and renal protective effects of phosphodiesterase-5 (PDE-5) inhibitors against ischemia-reperfusion injury have recently been demonstrated in animal studies. We evaluated the effect of pretreatment with the PDE-5 inhibitor zaprinast on warm renal ischemia in a rat model. Methods: Female Sprague-Dawley rats underwent concomitant right nephrectomy and left renal hilar occlusion for 30 minutes. Twelve animals were equally divided into three groups: Group 1 received no pharmacologic pretreatment, group 2 was pretreated with zaprinast 10 mg/kg, and group 3 was pretreated with zaprinast 20 mg/kg. Zaprinast was dissolved in 25% dimethyl sulfoxide and given as a single intraperitoneal injection 30 minutes before surgery. Serum blood urea nitrogen (BUN) and creatinine levels, histopathology, and TUNEL staining for apoptosis were assessed 24 hours postoperatively. Results: The mean creatinine level for groups 1, 2, and 3 was 0.73 mg/dL, 0.55 mg/dL, and 0.38 mg/dL, respectively. These values were not statistically different (P=0.099). The mean BUN levels of 35.8 mg/dL for group 1, 27.3 mg/dL for group 2, and 23.3 mg/dL for group 3 were also statistically similar (P=0.278). There were no objective differences in histopathologic evaluation or TUNEL staining between the groups. Conclusion: This study did not demonstrate a beneficial effect of zaprinast pretreatment on renal parameters after warm ischemic injury.

Original languageEnglish (US)
Pages (from-to)338-342
Number of pages5
JournalJournal of Endourology
Volume27
Issue number3
DOIs
StatePublished - Mar 1 2013

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ASJC Scopus subject areas

  • Urology

Cite this

Polcari, A. J., Farooq, A. V., Woods, M. E., Ripsch, M. S., Picken, M., Turk, T. M. T., & White, F. A. (2013). Effect of the phosphodiesterase-5 inhibitor zaprinast on ischemia-reperfusion injury in rats. Journal of Endourology, 27(3), 338-342. https://doi.org/10.1089/end.2012.0198