Effect of transferrin on alkaline phosphatase activity and collagen synthesis in osteoblastic cells derived from newborn mouse calvaria

Mauri Tsunoi, Yoshiyuki Hakeda, Noriyoshi Kurihara, Norihiko Maeda, Nobuo Utsumi, Masayoshi Kumegawa

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The effect of transferrin was tested on osteoblastic cells (clone MC3T3-E1) cultured in serum-free medium containing 1% bovine serum albumin (BSA). Transferrin (Tf) stimulated increases of protein content and protein synthesis, but not of DNA content and cell number, in the cells. This protein also increased alkaline phosphatase activity and collagen synthesis in combination with 1% BSA. Actinomycin D and cycloheximide inhibited alkaline phosphatase activity induced by Tf, suggesting that Tf may enhance de novo synthesis of the enzyme. These results indicate that Tf may be involved in differentiation of osteoblastic cells, but not in their proliferation, in vitro.

Original languageEnglish (US)
Pages (from-to)240-244
Number of pages5
JournalExperimental Cell Research
Volume153
Issue number1
DOIs
StatePublished - 1984
Externally publishedYes

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Transferrin
Skull
Alkaline Phosphatase
Collagen
Bovine Serum Albumin
Proteins
Serum-Free Culture Media
Dactinomycin
Cycloheximide
Cell Differentiation
Clone Cells
Cell Count
DNA
Enzymes

ASJC Scopus subject areas

  • Cell Biology

Cite this

Effect of transferrin on alkaline phosphatase activity and collagen synthesis in osteoblastic cells derived from newborn mouse calvaria. / Tsunoi, Mauri; Hakeda, Yoshiyuki; Kurihara, Noriyoshi; Maeda, Norihiko; Utsumi, Nobuo; Kumegawa, Masayoshi.

In: Experimental Cell Research, Vol. 153, No. 1, 1984, p. 240-244.

Research output: Contribution to journalArticle

Tsunoi, Mauri ; Hakeda, Yoshiyuki ; Kurihara, Noriyoshi ; Maeda, Norihiko ; Utsumi, Nobuo ; Kumegawa, Masayoshi. / Effect of transferrin on alkaline phosphatase activity and collagen synthesis in osteoblastic cells derived from newborn mouse calvaria. In: Experimental Cell Research. 1984 ; Vol. 153, No. 1. pp. 240-244.
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