Abstract
The effect of transferrin was tested on osteoblastic cells (clone MC3T3-E1) cultured in serum-free medium containing 1% bovine serum albumin (BSA). Transferrin (Tf) stimulated increases of protein content and protein synthesis, but not of DNA content and cell number, in the cells. This protein also increased alkaline phosphatase activity and collagen synthesis in combination with 1% BSA. Actinomycin D and cycloheximide inhibited alkaline phosphatase activity induced by Tf, suggesting that Tf may enhance de novo synthesis of the enzyme. These results indicate that Tf may be involved in differentiation of osteoblastic cells, but not in their proliferation, in vitro.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 240-244 |
| Number of pages | 5 |
| Journal | Experimental Cell Research |
| Volume | 153 |
| Issue number | 1 |
| DOIs | |
| State | Published - 1984 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Cell Biology
Cite this
Effect of transferrin on alkaline phosphatase activity and collagen synthesis in osteoblastic cells derived from newborn mouse calvaria. / Tsunoi, Mauri; Hakeda, Yoshiyuki; Kurihara, Noriyoshi; Maeda, Norihiko; Utsumi, Nobuo; Kumegawa, Masayoshi.
In: Experimental Cell Research, Vol. 153, No. 1, 1984, p. 240-244.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Effect of transferrin on alkaline phosphatase activity and collagen synthesis in osteoblastic cells derived from newborn mouse calvaria
AU - Tsunoi, Mauri
AU - Hakeda, Yoshiyuki
AU - Kurihara, Noriyoshi
AU - Maeda, Norihiko
AU - Utsumi, Nobuo
AU - Kumegawa, Masayoshi
PY - 1984
Y1 - 1984
N2 - The effect of transferrin was tested on osteoblastic cells (clone MC3T3-E1) cultured in serum-free medium containing 1% bovine serum albumin (BSA). Transferrin (Tf) stimulated increases of protein content and protein synthesis, but not of DNA content and cell number, in the cells. This protein also increased alkaline phosphatase activity and collagen synthesis in combination with 1% BSA. Actinomycin D and cycloheximide inhibited alkaline phosphatase activity induced by Tf, suggesting that Tf may enhance de novo synthesis of the enzyme. These results indicate that Tf may be involved in differentiation of osteoblastic cells, but not in their proliferation, in vitro.
AB - The effect of transferrin was tested on osteoblastic cells (clone MC3T3-E1) cultured in serum-free medium containing 1% bovine serum albumin (BSA). Transferrin (Tf) stimulated increases of protein content and protein synthesis, but not of DNA content and cell number, in the cells. This protein also increased alkaline phosphatase activity and collagen synthesis in combination with 1% BSA. Actinomycin D and cycloheximide inhibited alkaline phosphatase activity induced by Tf, suggesting that Tf may enhance de novo synthesis of the enzyme. These results indicate that Tf may be involved in differentiation of osteoblastic cells, but not in their proliferation, in vitro.
UR - http://www.scopus.com/inward/record.url?scp=0021215376&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0021215376&partnerID=8YFLogxK
U2 - 10.1016/0014-4827(84)90465-8
DO - 10.1016/0014-4827(84)90465-8
M3 - Article
C2 - 6734740
AN - SCOPUS:0021215376
VL - 153
SP - 240
EP - 244
JO - Experimental Cell Research
JF - Experimental Cell Research
SN - 0014-4827
IS - 1
ER -