Effect of twice-daily blue light treatment on matrix-rich biofilm development

Denise Lins De Sousa, Ramille Araújo Lima, Iriana Carla Zanin, Marlise I. Klein, Malvin N. Janal, Simone Duarte

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Background: The use of blue light has been proposed as a direct means of affecting local bacterial infections, however the use of blue light without a photosensitizer to prevent the biofilm development has not yet been explored. The aim of this study was to determine how the twice-daily treatment with blue light affects the development and composition of a matrix-rich biofilm. Methodology/Principal Findings: Biofilms of Streptococcus mutans UA159 were formed on saliva-coated hydroxyapatite discs for 5 days. The biofilms were exposed twice-daily to non-coherent blue light (Luma-Care; 420 nm) without a photosensitizer. The distance between the light and the sample was 1.0 cm; energy density of 72 J cm-2; and exposure time of 12 min 56 s. Positive and negative controls were twice-daily 0.12% chlorhexidine (CHX) and 0.89% NaCl, respectively. Biofilms were analyzed for bacterial viability, dry-weight, and extra (EPS-insoluble and soluble) and intracellular (IPS) polysaccharides. Variable pressure scanning electron microscopy and confocal scanning laser microscopy were used to check biofilm morphology and bacterial viability, respectively. When biofilms were exposed to twice-daily blue light, EPS-insoluble was reduced significantly more than in either control group (CHX and 0.89% NaCl). Bacterial viability and dry weight were also reduced relative to the negative control (0.89% NaCl) when the biofilms were treated with twice-daily blue light. Different morphology was also visible when the biofilms were treated with blue light. Conclusions: Twice-daily treatment with blue light without a photosensitizer is a promising mechanism for the inhibition of matrix-rich biofilm development.

Original languageEnglish (US)
Article numbere0131941
JournalPLoS One
Volume10
Issue number7
DOIs
StatePublished - Jul 31 2015
Externally publishedYes

Fingerprint

Biofilms
blue light
biofilm
Light
Microbial Viability
Photosensitizing Agents
chlorhexidine
Chlorhexidine
viability
Streptococcus mutans
Weights and Measures
hydroxyapatite
energy density
confocal laser scanning microscopy
Durapatite
saliva
bacterial infections
Saliva
Bacterial Infections
Confocal Microscopy

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

De Sousa, D. L., Lima, R. A., Zanin, I. C., Klein, M. I., Janal, M. N., & Duarte, S. (2015). Effect of twice-daily blue light treatment on matrix-rich biofilm development. PLoS One, 10(7), [e0131941]. https://doi.org/10.1371/journal.pone.0131941

Effect of twice-daily blue light treatment on matrix-rich biofilm development. / De Sousa, Denise Lins; Lima, Ramille Araújo; Zanin, Iriana Carla; Klein, Marlise I.; Janal, Malvin N.; Duarte, Simone.

In: PLoS One, Vol. 10, No. 7, e0131941, 31.07.2015.

Research output: Contribution to journalArticle

De Sousa, DL, Lima, RA, Zanin, IC, Klein, MI, Janal, MN & Duarte, S 2015, 'Effect of twice-daily blue light treatment on matrix-rich biofilm development', PLoS One, vol. 10, no. 7, e0131941. https://doi.org/10.1371/journal.pone.0131941
De Sousa, Denise Lins ; Lima, Ramille Araújo ; Zanin, Iriana Carla ; Klein, Marlise I. ; Janal, Malvin N. ; Duarte, Simone. / Effect of twice-daily blue light treatment on matrix-rich biofilm development. In: PLoS One. 2015 ; Vol. 10, No. 7.
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