Effect of varying oxygen concentrations on the proliferation of retinal microvascular cells in vitro

Paul Rosen, Mike Boulton, Patrick Moriarty, Asud Khaliq, David McLeod

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Microvascular fragments and pure cultures of either retinal microvascular endothelial cells, retinal pericytes or Tenon's capsule fibroblasts were grown on a gas permeable substrate while exposed to varying oxygen concentrations (5, 10, 20, 40 and 95% oxygen). For all three cell types cell proliferation was greatest under the lowest oxygen concentration and decreased as the oxygen concentration increased. The decrease in the proliferative ability of cells exposed to the higher oxygen concentrations could be reversed if the cells were returned to a normoxic environment. Endothelial cells were the most sensitive to changes in oxygen tensions showing a proliferative response after 24 hr exposure as opposed to 48 hr for pericytes and fibroblasts. These results suggest a direct mechanism by which the intra-vitreal and intra-retinal oxygen tension may influence both the development of preretinal new vessels (in response to retinal ischaemia) and also the regression of neovascularization following scatter photocoagulation or vitrectomy.

Original languageEnglish (US)
Pages (from-to)597-601
Number of pages5
JournalExperimental Eye Research
Volume53
Issue number5
DOIs
StatePublished - 1991
Externally publishedYes

Fingerprint

Oxygen
Pericytes
Tenon Capsule
Endothelial Cells
Fibroblasts
Light Coagulation
Vitrectomy
In Vitro Techniques
Ischemia
Gases
Cell Proliferation

Keywords

  • fibroblasts
  • hyperoxia
  • hypoxia
  • normoxia
  • oxygen
  • proliferation
  • retinal microvascular endothelial cells
  • retinal pericytes

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems

Cite this

Effect of varying oxygen concentrations on the proliferation of retinal microvascular cells in vitro. / Rosen, Paul; Boulton, Mike; Moriarty, Patrick; Khaliq, Asud; McLeod, David.

In: Experimental Eye Research, Vol. 53, No. 5, 1991, p. 597-601.

Research output: Contribution to journalArticle

Rosen, Paul ; Boulton, Mike ; Moriarty, Patrick ; Khaliq, Asud ; McLeod, David. / Effect of varying oxygen concentrations on the proliferation of retinal microvascular cells in vitro. In: Experimental Eye Research. 1991 ; Vol. 53, No. 5. pp. 597-601.
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