Effect of violet-blue light on Streptococcus mutans-induced enamel demineralization

Grace Gomez Felix Gomez, Frank Lippert, Masatoshi Ando, Andrea Ferreira Zandona, George J. Eckert, Richard Gregory

Research output: Contribution to journalArticle

Abstract

Background: This in vitro study determined the effectiveness of violet-blue light (405 nm) on inhibiting Streptococcus mutans-induced enamel demineralization. Materials and Methods: S. mutans UA159 biofilm was grown on human enamel specimens for 13 h in 5% CO 2 at 37 °C with/without 1% sucrose. Wet biofilm was treated twice daily with violet-blue light for five minutes over five days. A six-hour reincubation was included daily between treatments excluding the final day. Biofilms were harvested and colony forming units (CFU) were quantitated. Lesion depth (L) and mineral loss (∆Z) were quantified using transverse microradiography (TMR). Quantitative light-induced fluorescence Biluminator (QLF-D) was used to determine mean fluorescence loss. Data were analyzed using one-way analysis of variance (ANOVA) to compare differences in means. Results: The results demonstrated a significant reduction in CFUs between treated and non-treated groups grown with/without 1% sucrose. ∆Z was significantly reduced for specimens exposed to biofilms grown without sucrose with violet-blue light. There was only a trend on reduction of ∆Z with sucrose and with L on both groups. There were no differences in fluorescence-derived parameters between the groups. Conclusions: Within the limitations of the study, the results indicate that violet-blue light can serve as an adjunct prophylactic treatment for reducing S. mutans biofilm formation and enamel mineral loss.

Original languageEnglish (US)
Article number6
JournalDentistry Journal
Volume6
Issue number2
DOIs
StatePublished - Jan 1 2018

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Viola
Streptococcus mutans
Dental Enamel
Biofilms
Sucrose
Light
Fluorescence
Minerals
Microradiography
Carbon Monoxide
Analysis of Variance
Stem Cells

Keywords

  • Dental caries
  • Phototherapy
  • Streptococcus mutans
  • Violet-blue light

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Effect of violet-blue light on Streptococcus mutans-induced enamel demineralization. / Felix Gomez, Grace Gomez; Lippert, Frank; Ando, Masatoshi; Zandona, Andrea Ferreira; Eckert, George J.; Gregory, Richard.

In: Dentistry Journal, Vol. 6, No. 2, 6, 01.01.2018.

Research output: Contribution to journalArticle

Felix Gomez, Grace Gomez ; Lippert, Frank ; Ando, Masatoshi ; Zandona, Andrea Ferreira ; Eckert, George J. ; Gregory, Richard. / Effect of violet-blue light on Streptococcus mutans-induced enamel demineralization. In: Dentistry Journal. 2018 ; Vol. 6, No. 2.
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abstract = "Background: This in vitro study determined the effectiveness of violet-blue light (405 nm) on inhibiting Streptococcus mutans-induced enamel demineralization. Materials and Methods: S. mutans UA159 biofilm was grown on human enamel specimens for 13 h in 5{\%} CO 2 at 37 °C with/without 1{\%} sucrose. Wet biofilm was treated twice daily with violet-blue light for five minutes over five days. A six-hour reincubation was included daily between treatments excluding the final day. Biofilms were harvested and colony forming units (CFU) were quantitated. Lesion depth (L) and mineral loss (∆Z) were quantified using transverse microradiography (TMR). Quantitative light-induced fluorescence Biluminator (QLF-D) was used to determine mean fluorescence loss. Data were analyzed using one-way analysis of variance (ANOVA) to compare differences in means. Results: The results demonstrated a significant reduction in CFUs between treated and non-treated groups grown with/without 1{\%} sucrose. ∆Z was significantly reduced for specimens exposed to biofilms grown without sucrose with violet-blue light. There was only a trend on reduction of ∆Z with sucrose and with L on both groups. There were no differences in fluorescence-derived parameters between the groups. Conclusions: Within the limitations of the study, the results indicate that violet-blue light can serve as an adjunct prophylactic treatment for reducing S. mutans biofilm formation and enamel mineral loss.",
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