Effect of whole-body hyperthermia and cyclophosphamide on natural killer cell activity in murine erythroleukemia

R. N. Shen, N. B. Hornback, H. Shidnia, L. Lu, Hal Broxmeyer, Z. Brahmi

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Mice infected with the polycythemia-inducing strain of Friend virus complex (FVC-P) develop a fatal erythroid disease similar in some respects to leukemia. Six- to eight-week-old DBA/2 female mice were injected i.v. with 0.5 ml of a virus suspension containing approximately 5 x 104 plaque-forming units and 5 x 103 spleen focus-forming units. Four treatment regimens were begun 3 days postinjection: (a) no treatment; (b) whole-body hyperthermia (WBH) alone; (c) cyclophosphamide (CY) alone; (d) WBH combined with CY. WBH treatment utilized a microwave generator operating at 2450 MHz. The i.p. temperature of the mice receiving WBH was maintained at 39.5-40°C for 30 min. The CY was given i.p. at a dosage of 20 mg/kg of body weight. The various treatments, CY, WBH, CY + WBH were given once a week for 2 weeks. Natural killer cell activity was examined in all four groups of mice and was found to be significantly higher in the animals treated with WBH or CY. Our results show that WBH, either alone or in combination with CY, can prolong the lifespan of mice infected with lethal dosages of the FVC-P, possibly via a mechanism involving natural killer cells.

Original languageEnglish
Pages (from-to)4561-4563
Number of pages3
JournalCancer Research
Volume48
Issue number16
StatePublished - 1988

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Leukemia, Erythroblastic, Acute
Natural Killer Cells
Cyclophosphamide
Fever
Friend murine leukemia virus
Polycythemia
Microwaves
Suspensions
Leukemia
Spleen
Body Weight
Viruses
Temperature

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Shen, R. N., Hornback, N. B., Shidnia, H., Lu, L., Broxmeyer, H., & Brahmi, Z. (1988). Effect of whole-body hyperthermia and cyclophosphamide on natural killer cell activity in murine erythroleukemia. Cancer Research, 48(16), 4561-4563.

Effect of whole-body hyperthermia and cyclophosphamide on natural killer cell activity in murine erythroleukemia. / Shen, R. N.; Hornback, N. B.; Shidnia, H.; Lu, L.; Broxmeyer, Hal; Brahmi, Z.

In: Cancer Research, Vol. 48, No. 16, 1988, p. 4561-4563.

Research output: Contribution to journalArticle

Shen, RN, Hornback, NB, Shidnia, H, Lu, L, Broxmeyer, H & Brahmi, Z 1988, 'Effect of whole-body hyperthermia and cyclophosphamide on natural killer cell activity in murine erythroleukemia', Cancer Research, vol. 48, no. 16, pp. 4561-4563.
Shen, R. N. ; Hornback, N. B. ; Shidnia, H. ; Lu, L. ; Broxmeyer, Hal ; Brahmi, Z. / Effect of whole-body hyperthermia and cyclophosphamide on natural killer cell activity in murine erythroleukemia. In: Cancer Research. 1988 ; Vol. 48, No. 16. pp. 4561-4563.
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