Effects in Premature Infants of Normalizing Breath h2Concentrations with CO2: Increased h2Concentration and Reduced Interaliquot Variation

C. Lawrence Kien, Edward A. Liechty, David Z. Myerberg, Martha D. Mullett

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Interval sampling of expired breath samples from the anterior nares is a feasible and noninvasive method for detecting elevated breath H2 and thus colonic carbohydrate fermentation, especially in nontherapeutic research studies of healthy premature infants. However, there may be a risk of falsely low breath H2 concentration and an unacceptable experimental error due to contamination with room air as well as with dead space air. We studied ten premature infants (28-32 week gestational age and 2-4 weeks postnatal age) who were receiving either a proprietary formula containing equal proportions of lactose and glucose polymer, or a similar formula in which lactose was the sole carbohydrate. In 70 breath samples (obtained 30-180 min after feeding) we assessed the coefficient of variation in breath hydrogen concentration among three aliquots obtained over a 3-5 min interval. Breath was collected from the anterior nares. The interaliquot coefficient of variation averaged 11% when expressed as parts per million per 5% CO2, compared to 19% when expressed as parts per million (Wilcoxon, p <0.001). Mean breath hydrogen concentration at each time period using the former method (parts per million per 5% CO2) was about 100% higher than when using the latter method. Although ventilation rate can alter alveolar CO2, normalizing for CO2 concentration reduces a major source of experimental error.

Original languageEnglish (US)
Pages (from-to)286-289
Number of pages4
JournalJournal of Pediatric Gastroenterology and Nutrition
Volume6
Issue number2
StatePublished - 1987
Externally publishedYes

Fingerprint

Premature Infants
carbon dioxide
Lactose
Nontherapeutic Human Experimentation
Hydrogen
Air
Carbohydrates
hydrogen
lactose
Glucans
carbohydrates
air
Gestational Age
Fermentation
Ventilation
gestational age
Research Design
sampling
polymers
methodology

Keywords

  • Breath hy-drogen concentration
  • Interaliquot variation
  • Premature infants

ASJC Scopus subject areas

  • Gastroenterology
  • Pediatrics, Perinatology, and Child Health
  • Food Science
  • Medicine (miscellaneous)
  • Histology

Cite this

Effects in Premature Infants of Normalizing Breath h2Concentrations with CO2 : Increased h2Concentration and Reduced Interaliquot Variation. / Lawrence Kien, C.; Liechty, Edward A.; Myerberg, David Z.; Mullett, Martha D.

In: Journal of Pediatric Gastroenterology and Nutrition, Vol. 6, No. 2, 1987, p. 286-289.

Research output: Contribution to journalArticle

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