Effects of 1-oleoyl-2-acetyl glycerol are distinct from those of phorbol ester in rat pancreatic acini

J. Scott Brockenbrough, Murray Korc

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

1-oleoyl-2-acetyl glycerol (OAG), a potent activator of protein kinase C, inhibited the binding of 125I-labelled epidermal growth factor (EGF) in isolated rat pancreatic acini. Unlike cholecystokinin-octapeptide (CCK8) and the C-kinase activator 12-0-tetradecanoyl phorbol-13-acetate (TPA), two inhibitors of 125I-EGF endocytosis in the pancreas, OAG had no effect on the distribution of bound ligand between the cell surface and intracellular compartments. Unlike TPA, OAG failed to potentiate the inhibitory effects of the calcium ionophore A23187 on 125I-EGF cell-associated radioactivity and had no effect on either basal or carbachol-stimulated amylase release in acini. These data suggest that the actions of the synthetic diacylglycerol OAG are not fully equivalent with the action of other known activators of protein kinase C in the pancreatic acinar cell.

Original languageEnglish (US)
Pages (from-to)1625-1631
Number of pages7
JournalLife Sciences
Volume40
Issue number16
DOIs
StatePublished - Apr 20 1987
Externally publishedYes

Fingerprint

Phorbol Esters
Rats
Epidermal Growth Factor
Protein Kinase C
Acetates
Sincalide
Calcium Ionophores
Acinar Cells
Diglycerides
Radioactivity
Calcimycin
Carbachol
Amylases
Endocytosis
Pancreas
Phosphotransferases
Ligands
1-oleoyl-2-acetylglycerol
phorbol

ASJC Scopus subject areas

  • Pharmacology

Cite this

Effects of 1-oleoyl-2-acetyl glycerol are distinct from those of phorbol ester in rat pancreatic acini. / Scott Brockenbrough, J.; Korc, Murray.

In: Life Sciences, Vol. 40, No. 16, 20.04.1987, p. 1625-1631.

Research output: Contribution to journalArticle

Scott Brockenbrough, J. ; Korc, Murray. / Effects of 1-oleoyl-2-acetyl glycerol are distinct from those of phorbol ester in rat pancreatic acini. In: Life Sciences. 1987 ; Vol. 40, No. 16. pp. 1625-1631.
@article{61f7d318354748c09eedb835e8b9c402,
title = "Effects of 1-oleoyl-2-acetyl glycerol are distinct from those of phorbol ester in rat pancreatic acini",
abstract = "1-oleoyl-2-acetyl glycerol (OAG), a potent activator of protein kinase C, inhibited the binding of 125I-labelled epidermal growth factor (EGF) in isolated rat pancreatic acini. Unlike cholecystokinin-octapeptide (CCK8) and the C-kinase activator 12-0-tetradecanoyl phorbol-13-acetate (TPA), two inhibitors of 125I-EGF endocytosis in the pancreas, OAG had no effect on the distribution of bound ligand between the cell surface and intracellular compartments. Unlike TPA, OAG failed to potentiate the inhibitory effects of the calcium ionophore A23187 on 125I-EGF cell-associated radioactivity and had no effect on either basal or carbachol-stimulated amylase release in acini. These data suggest that the actions of the synthetic diacylglycerol OAG are not fully equivalent with the action of other known activators of protein kinase C in the pancreatic acinar cell.",
author = "{Scott Brockenbrough}, J. and Murray Korc",
year = "1987",
month = "4",
day = "20",
doi = "10.1016/0024-3205(87)90129-9",
language = "English (US)",
volume = "40",
pages = "1625--1631",
journal = "Life Sciences",
issn = "0024-3205",
publisher = "Elsevier Inc.",
number = "16",

}

TY - JOUR

T1 - Effects of 1-oleoyl-2-acetyl glycerol are distinct from those of phorbol ester in rat pancreatic acini

AU - Scott Brockenbrough, J.

AU - Korc, Murray

PY - 1987/4/20

Y1 - 1987/4/20

N2 - 1-oleoyl-2-acetyl glycerol (OAG), a potent activator of protein kinase C, inhibited the binding of 125I-labelled epidermal growth factor (EGF) in isolated rat pancreatic acini. Unlike cholecystokinin-octapeptide (CCK8) and the C-kinase activator 12-0-tetradecanoyl phorbol-13-acetate (TPA), two inhibitors of 125I-EGF endocytosis in the pancreas, OAG had no effect on the distribution of bound ligand between the cell surface and intracellular compartments. Unlike TPA, OAG failed to potentiate the inhibitory effects of the calcium ionophore A23187 on 125I-EGF cell-associated radioactivity and had no effect on either basal or carbachol-stimulated amylase release in acini. These data suggest that the actions of the synthetic diacylglycerol OAG are not fully equivalent with the action of other known activators of protein kinase C in the pancreatic acinar cell.

AB - 1-oleoyl-2-acetyl glycerol (OAG), a potent activator of protein kinase C, inhibited the binding of 125I-labelled epidermal growth factor (EGF) in isolated rat pancreatic acini. Unlike cholecystokinin-octapeptide (CCK8) and the C-kinase activator 12-0-tetradecanoyl phorbol-13-acetate (TPA), two inhibitors of 125I-EGF endocytosis in the pancreas, OAG had no effect on the distribution of bound ligand between the cell surface and intracellular compartments. Unlike TPA, OAG failed to potentiate the inhibitory effects of the calcium ionophore A23187 on 125I-EGF cell-associated radioactivity and had no effect on either basal or carbachol-stimulated amylase release in acini. These data suggest that the actions of the synthetic diacylglycerol OAG are not fully equivalent with the action of other known activators of protein kinase C in the pancreatic acinar cell.

UR - http://www.scopus.com/inward/record.url?scp=0023117925&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023117925&partnerID=8YFLogxK

U2 - 10.1016/0024-3205(87)90129-9

DO - 10.1016/0024-3205(87)90129-9

M3 - Article

C2 - 2436020

AN - SCOPUS:0023117925

VL - 40

SP - 1625

EP - 1631

JO - Life Sciences

JF - Life Sciences

SN - 0024-3205

IS - 16

ER -