Effects of 1,25-dihydroxy-vitamin D3 on phosphate accumulation by myoblasts

B. Teresita, R. Boland

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

The effects of 1,25-dihydroxy-Vitamin D3 on phosphate uptake by cultured chick embryonic muscle cells were investigated. Preincubation of primary myoblast cultures during 4-24 hours with physiological levels of 1,25(OH)2D3 resulted in a significant stimulation of velocity and total capacity of phosphate accumulation by the cells. Maximal responses were obtained at 8 hours of treatment with the sterol. In agreement with previous studies, 25-hydroxy-Vitamin D3 also stimulated myoblast phosphate uptake. 24,25-dihydroxy-Vitamin D3 and vitamin D3 were ineffective. Evidence was obtained that indicated that 1,25(OH)2D3 affects the Na+-linked component of muscle cell phosphate uptake through a mechanism dependent on 'de movo' protein and RNA synthesis.

Original languageEnglish (US)
Pages (from-to)113-116
Number of pages4
JournalHormone and Metabolic Research
Volume23
Issue number3
StatePublished - Mar 27 1991
Externally publishedYes

Keywords

  • 1,25-dihydroxy-Vitamin D-vitamin D
  • Muscle cells
  • Myoblasts
  • Phosphate accumulation

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Endocrinology
  • Clinical Biochemistry
  • Biochemistry, medical

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