Effects of 250 mg% ethanol on monoamine and amino acid release from rat striatal slices

J. M. Murphy, S. D. Cunningham, W. J. McBride

Research output: Contribution to journalArticle

22 Scopus citations

Abstract

A single IP injection of 2.5 g ethanol/kg body weight into the rat increased the striatal levels of 3,4-dihydroxyphenylacetic acid (DOPAC) and homovanillic acid (HVA) one hour later to 133 and 141% of control values, respectively. Blood alcohol concentrations at this time were approximately 250 mg%. The increased striatal tissue levels of DOPAC and HVA found after IP administration did not appear to be due to a direct effect of ethanol on the efflux of these two metabolites or on the release of dopamine (DA) since in vitro studies with striatal slices demonstrated that 250 mg% ethanol had no effect on the endogenous release of DOPAC, HVA, or DA. However, ethanol did enhance the K+-stimulated, Ca2+-dependent release of glutamate and aspartate from striatal slices to 168 and 214% of control values, respectively. The release of glutamate and aspartate from slices of midbrain (minus colliculi) was also increased by 250 mg% ethanol. On the other hand, the release of GABA, NE and 5-HT did not appear to be significantly altered by 250 mg% ethanol. The in vitro findings have led to the hypothesis that the elevated DOPAC and HVA levels observed in the striatum following an acute IP injection of 2.5 g/kg of ethanol are due to increased release of DA produced by the excitatory actions of glutamate (and/or aspartate) on dopaminergic neurons.

Original languageEnglish (US)
Pages (from-to)439-442
Number of pages4
JournalBrain Research Bulletin
Volume14
Issue number5
DOIs
StatePublished - May 1985

Keywords

  • DOPAC
  • Dopamine
  • Ethanol
  • GABA
  • Glutamate Aspartate
  • HVA
  • Norepinephrine
  • Release
  • Serotonin
  • Striatum

ASJC Scopus subject areas

  • Neuroscience(all)

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