Effects of 250 mg% ethanol on monoamine and amino acid release from rat striatal slices

J. M. Murphy, S. D. Cunningham, W. J. McBride

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

A single IP injection of 2.5 g ethanol/kg body weight into the rat increased the striatal levels of 3,4-dihydroxyphenylacetic acid (DOPAC) and homovanillic acid (HVA) one hour later to 133 and 141% of control values, respectively. Blood alcohol concentrations at this time were approximately 250 mg%. The increased striatal tissue levels of DOPAC and HVA found after IP administration did not appear to be due to a direct effect of ethanol on the efflux of these two metabolites or on the release of dopamine (DA) since in vitro studies with striatal slices demonstrated that 250 mg% ethanol had no effect on the endogenous release of DOPAC, HVA, or DA. However, ethanol did enhance the K+-stimulated, Ca2+-dependent release of glutamate and aspartate from striatal slices to 168 and 214% of control values, respectively. The release of glutamate and aspartate from slices of midbrain (minus colliculi) was also increased by 250 mg% ethanol. On the other hand, the release of GABA, NE and 5-HT did not appear to be significantly altered by 250 mg% ethanol. The in vitro findings have led to the hypothesis that the elevated DOPAC and HVA levels observed in the striatum following an acute IP injection of 2.5 g/kg of ethanol are due to increased release of DA produced by the excitatory actions of glutamate (and/or aspartate) on dopaminergic neurons.

Original languageEnglish
Pages (from-to)439-442
Number of pages4
JournalBrain Research Bulletin
Volume14
Issue number5
DOIs
StatePublished - 1985

Fingerprint

Corpus Striatum
Ethanol
3,4-Dihydroxyphenylacetic Acid
Homovanillic Acid
Amino Acids
Aspartic Acid
Glutamic Acid
Dopamine
Injections
Dopaminergic Neurons
Mesencephalon
gamma-Aminobutyric Acid
Serotonin
Body Weight

Keywords

  • DOPAC
  • Dopamine
  • Ethanol
  • GABA
  • Glutamate Aspartate
  • HVA
  • Norepinephrine
  • Release
  • Serotonin
  • Striatum

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Effects of 250 mg% ethanol on monoamine and amino acid release from rat striatal slices. / Murphy, J. M.; Cunningham, S. D.; McBride, W. J.

In: Brain Research Bulletin, Vol. 14, No. 5, 1985, p. 439-442.

Research output: Contribution to journalArticle

Murphy, J. M. ; Cunningham, S. D. ; McBride, W. J. / Effects of 250 mg% ethanol on monoamine and amino acid release from rat striatal slices. In: Brain Research Bulletin. 1985 ; Vol. 14, No. 5. pp. 439-442.
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abstract = "A single IP injection of 2.5 g ethanol/kg body weight into the rat increased the striatal levels of 3,4-dihydroxyphenylacetic acid (DOPAC) and homovanillic acid (HVA) one hour later to 133 and 141{\%} of control values, respectively. Blood alcohol concentrations at this time were approximately 250 mg{\%}. The increased striatal tissue levels of DOPAC and HVA found after IP administration did not appear to be due to a direct effect of ethanol on the efflux of these two metabolites or on the release of dopamine (DA) since in vitro studies with striatal slices demonstrated that 250 mg{\%} ethanol had no effect on the endogenous release of DOPAC, HVA, or DA. However, ethanol did enhance the K+-stimulated, Ca2+-dependent release of glutamate and aspartate from striatal slices to 168 and 214{\%} of control values, respectively. The release of glutamate and aspartate from slices of midbrain (minus colliculi) was also increased by 250 mg{\%} ethanol. On the other hand, the release of GABA, NE and 5-HT did not appear to be significantly altered by 250 mg{\%} ethanol. The in vitro findings have led to the hypothesis that the elevated DOPAC and HVA levels observed in the striatum following an acute IP injection of 2.5 g/kg of ethanol are due to increased release of DA produced by the excitatory actions of glutamate (and/or aspartate) on dopaminergic neurons.",
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