Effects of 5 hydroxytryptophan on serotonin in nerve endings

W. J. McBride, M. H. Aprison, J. N. Hingtgen

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Preparations of synaptosomes (P2) from the telencephalon and from the diencephalon plus optic lobes of the pigeon and from the telencephalon of the rat were used to study the effects of 5 hydroxytryptophan (5 HTP) on the levels of serotonin (5 HT) in nerve endings and the release of 5 HT from nerve endings. The levels of 5 HT were significantly higher (3.21 ± 0.35 nmol/g original tissue weight) in the P2 fraction isolated from the telencephalon of pigeons given im injections of 50 mg/kg of D,L 5 HTP in comparison to control values (1.42 ± 0.07). A similar 2 fold increase was observed with the P2 fraction isolated from the diencephalon plus optic lobes. In addition, the levels of 5 HTP and 5 hydroxyindole acetic acid also increased significantly in these P2 fractions isolated from pigeons given D,L 5 HTP injections in comparison to values obtained for pigeons given saline injections. In vitro studies using preparations of synaptosomes (from both pigeon and rat) labelled with [3H]5 HT indicated that 0.10 mM L 5 HTP increased the release of [3H]5 HT 2 fold over control values. A concentration as low as 0.001 mM L 5 HTP was tested on the P2 fraction from the telencephalon of the pigeon and was found to significantly increase the release of [3H]5 HT over control values. This effect by L 5 HTP was blocked if a decarboxylase inhibitor was added to the medium. L 5 HTP at a concentration of 1.5 mM had no apparent effect on the release of [3H]norepinephrine or [3H]dopamine from synaptosomes prepared from the telencephalon of the rat or pigeon. The results are discussed in terms of the role of serotonin in producing certain types of behavioral depressions exhibited by pigeons and rats given injections of 5 HTP.

Original languageEnglish
Pages (from-to)385-391
Number of pages7
JournalJournal of Neurochemistry
Volume23
Issue number2
StatePublished - 1974

Fingerprint

5-Hydroxytryptophan
Nerve Endings
Columbidae
Serotonin
Telencephalon
Synaptosomes
Rats
Diencephalon
Injections
Optics
Carboxy-Lyases
Acetic Acid
Dopamine
Norepinephrine
Tissue
Weights and Measures

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

McBride, W. J., Aprison, M. H., & Hingtgen, J. N. (1974). Effects of 5 hydroxytryptophan on serotonin in nerve endings. Journal of Neurochemistry, 23(2), 385-391.

Effects of 5 hydroxytryptophan on serotonin in nerve endings. / McBride, W. J.; Aprison, M. H.; Hingtgen, J. N.

In: Journal of Neurochemistry, Vol. 23, No. 2, 1974, p. 385-391.

Research output: Contribution to journalArticle

McBride, WJ, Aprison, MH & Hingtgen, JN 1974, 'Effects of 5 hydroxytryptophan on serotonin in nerve endings', Journal of Neurochemistry, vol. 23, no. 2, pp. 385-391.
McBride WJ, Aprison MH, Hingtgen JN. Effects of 5 hydroxytryptophan on serotonin in nerve endings. Journal of Neurochemistry. 1974;23(2):385-391.
McBride, W. J. ; Aprison, M. H. ; Hingtgen, J. N. / Effects of 5 hydroxytryptophan on serotonin in nerve endings. In: Journal of Neurochemistry. 1974 ; Vol. 23, No. 2. pp. 385-391.
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