Effects of a chemotactic factor, N-formylmethionyl peptide, on adherence, superoxide anion generation, phagocytosis, and microtubule assembly of human polymorphonuclear leukocytes

Laurence A. Boxer, Mervin Yoder, Stephen Bonsib, Michael Schmidt, Peter Ho, Ralph Jersild, Robert L. Baehner

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Abstract

FMLP promoted microtubule assembly in PMNs at concentrations which were chemotactic for the cells. At higher concentrations than those required for chemotaxis, FMLP enhanced the adherence of PMNs to nylon glass fibers. With colchicine, PMN adherence was inhibited, but upon exposure to FMLP, PMN adherence could be restored. The effect of FMLP on PMN adherence was transitory and was no longer evident by 5 min. At concentrations similar to those employed in the adherence studies, FMLP induced the cells to briefly generate O-2, since ferricytochrome C reduction was no longer evident by 5 min. Pretreatment of the PMNs with cytochalasin B enhanced the release of O-2 by PMNs exposed to FMLP. On the other hand, there was no effect of FMLP on phagocytosis of C3-coated particles. These results suggest that FMLP induces responsive cells to develop a hyperadherent plasma membrane which is largely independent of microtubule control. Since oligopeptides similar of FMLP are formed in bacteria, it is likely that the action of N-formylated peptides is important in regulating the inflammatory response.

Original languageEnglish
Pages (from-to)506-514
Number of pages9
JournalJournal of Laboratory and Clinical Medicine
Volume93
Issue number3
StatePublished - 1979

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N-Formylmethionine Leucyl-Phenylalanine
Oligopeptides
Cytochalasin B
Nylons
Chemotactic Factors
Colchicine
Cell membranes
Phagocytosis
Microtubules
Superoxides
Bacteria
Neutrophils
Chemotaxis
Cell Membrane
fiberglass

ASJC Scopus subject areas

  • Medicine(all)
  • Pathology and Forensic Medicine

Cite this

Effects of a chemotactic factor, N-formylmethionyl peptide, on adherence, superoxide anion generation, phagocytosis, and microtubule assembly of human polymorphonuclear leukocytes. / Boxer, Laurence A.; Yoder, Mervin; Bonsib, Stephen; Schmidt, Michael; Ho, Peter; Jersild, Ralph; Baehner, Robert L.

In: Journal of Laboratory and Clinical Medicine, Vol. 93, No. 3, 1979, p. 506-514.

Research output: Contribution to journalArticle

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AU - Boxer, Laurence A.

AU - Yoder, Mervin

AU - Bonsib, Stephen

AU - Schmidt, Michael

AU - Ho, Peter

AU - Jersild, Ralph

AU - Baehner, Robert L.

PY - 1979

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N2 - FMLP promoted microtubule assembly in PMNs at concentrations which were chemotactic for the cells. At higher concentrations than those required for chemotaxis, FMLP enhanced the adherence of PMNs to nylon glass fibers. With colchicine, PMN adherence was inhibited, but upon exposure to FMLP, PMN adherence could be restored. The effect of FMLP on PMN adherence was transitory and was no longer evident by 5 min. At concentrations similar to those employed in the adherence studies, FMLP induced the cells to briefly generate O-2, since ferricytochrome C reduction was no longer evident by 5 min. Pretreatment of the PMNs with cytochalasin B enhanced the release of O-2 by PMNs exposed to FMLP. On the other hand, there was no effect of FMLP on phagocytosis of C3-coated particles. These results suggest that FMLP induces responsive cells to develop a hyperadherent plasma membrane which is largely independent of microtubule control. Since oligopeptides similar of FMLP are formed in bacteria, it is likely that the action of N-formylated peptides is important in regulating the inflammatory response.

AB - FMLP promoted microtubule assembly in PMNs at concentrations which were chemotactic for the cells. At higher concentrations than those required for chemotaxis, FMLP enhanced the adherence of PMNs to nylon glass fibers. With colchicine, PMN adherence was inhibited, but upon exposure to FMLP, PMN adherence could be restored. The effect of FMLP on PMN adherence was transitory and was no longer evident by 5 min. At concentrations similar to those employed in the adherence studies, FMLP induced the cells to briefly generate O-2, since ferricytochrome C reduction was no longer evident by 5 min. Pretreatment of the PMNs with cytochalasin B enhanced the release of O-2 by PMNs exposed to FMLP. On the other hand, there was no effect of FMLP on phagocytosis of C3-coated particles. These results suggest that FMLP induces responsive cells to develop a hyperadherent plasma membrane which is largely independent of microtubule control. Since oligopeptides similar of FMLP are formed in bacteria, it is likely that the action of N-formylated peptides is important in regulating the inflammatory response.

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