Effects of acute administration of ethanol on cerebral glucose utilization in adult alcohol-preferring and alcohol-nonpreferring rats

Wendy N. Strother, William J. McBride, Lawrence Lumeng, Ting Kai Li

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Local cerebral glucose utilization (LCGU) rates, as determined by the [14C]-2-deoxyglucose (2-DG) technique, were examined after acute ethanol administration within selected brain regions of alcohol-preferring (P) and alcohol-nonpreferring (NP) rats. Adult male P and NP rats were injected with saline, 0.25 g/kg, or 1.0 g/kg ethanol, intraperitoneally (ip), 10 min before an intravenous bolus of [14C]2-DG (125 μCi/kg). Timed arterial blood samples were collected over 45 min and assayed for plasma glucose, ethanol, and [14C]2-DG levels. Image densities were determined using quantitative autoradiography and LCGU values calculated. Data were collected from several key limbic, basal ganglionic, cortical, and subcortical structures. Low-dose ethanol (0.25 g/kg) significantly decreased LCGU rates in several brain regions including the medial prefrontal cortex, olfactory tubercles, and the CA1 subregion of the hippocampus of P rats. Low-dose ethanol had no significant effects on LCGU rates in the NP rats. Moderate-dose ethanol (1.0 g/kg) also significantly lowered LCGU rates in many brain regions of P rats, including key limbic structures, such as the medial prefrontal cortex, olfactory tubercles, ventral tegmental area, basolateral nucleus of the amygdala, lateral septum, and ventral pallidum. Moderate-dose ethanol also significantly lowered LCGU rates in the medial prefrontal cortex as well as in the habenula of NP rats. All other regions were unaffected in the NP rats. These findings support the suggestion that certain central nervous system regions of P rats may be more sensitive than those of NP rats to the effects of low to intermediate doses of ethanol.

Original languageEnglish
Pages (from-to)119-128
Number of pages10
JournalAlcohol
Volume35
Issue number2
DOIs
StatePublished - Feb 2005

Fingerprint

Rats
Ethanol
utilization
alcohol
Alcohols
Glucose
brain
Deoxyglucose
Prefrontal Cortex
Brain
Habenula
Ventral Tegmental Area
Neurology
Amygdala
Autoradiography
Hippocampus
Blood
Central Nervous System
Plasmas
Values

Keywords

  • Alcohol-nonpreferring rats
  • Alcohol-preferring rats
  • Ethanol effects
  • Local cerebral glucose utilization

ASJC Scopus subject areas

  • Biochemistry
  • Medicine(all)
  • Behavioral Neuroscience
  • Neuroscience(all)
  • Toxicology
  • Health(social science)

Cite this

Effects of acute administration of ethanol on cerebral glucose utilization in adult alcohol-preferring and alcohol-nonpreferring rats. / Strother, Wendy N.; McBride, William J.; Lumeng, Lawrence; Li, Ting Kai.

In: Alcohol, Vol. 35, No. 2, 02.2005, p. 119-128.

Research output: Contribution to journalArticle

Strother, Wendy N. ; McBride, William J. ; Lumeng, Lawrence ; Li, Ting Kai. / Effects of acute administration of ethanol on cerebral glucose utilization in adult alcohol-preferring and alcohol-nonpreferring rats. In: Alcohol. 2005 ; Vol. 35, No. 2. pp. 119-128.
@article{cbf1b2462d2f41c79e6c42e54e409e3b,
title = "Effects of acute administration of ethanol on cerebral glucose utilization in adult alcohol-preferring and alcohol-nonpreferring rats",
abstract = "Local cerebral glucose utilization (LCGU) rates, as determined by the [14C]-2-deoxyglucose (2-DG) technique, were examined after acute ethanol administration within selected brain regions of alcohol-preferring (P) and alcohol-nonpreferring (NP) rats. Adult male P and NP rats were injected with saline, 0.25 g/kg, or 1.0 g/kg ethanol, intraperitoneally (ip), 10 min before an intravenous bolus of [14C]2-DG (125 μCi/kg). Timed arterial blood samples were collected over 45 min and assayed for plasma glucose, ethanol, and [14C]2-DG levels. Image densities were determined using quantitative autoradiography and LCGU values calculated. Data were collected from several key limbic, basal ganglionic, cortical, and subcortical structures. Low-dose ethanol (0.25 g/kg) significantly decreased LCGU rates in several brain regions including the medial prefrontal cortex, olfactory tubercles, and the CA1 subregion of the hippocampus of P rats. Low-dose ethanol had no significant effects on LCGU rates in the NP rats. Moderate-dose ethanol (1.0 g/kg) also significantly lowered LCGU rates in many brain regions of P rats, including key limbic structures, such as the medial prefrontal cortex, olfactory tubercles, ventral tegmental area, basolateral nucleus of the amygdala, lateral septum, and ventral pallidum. Moderate-dose ethanol also significantly lowered LCGU rates in the medial prefrontal cortex as well as in the habenula of NP rats. All other regions were unaffected in the NP rats. These findings support the suggestion that certain central nervous system regions of P rats may be more sensitive than those of NP rats to the effects of low to intermediate doses of ethanol.",
keywords = "Alcohol-nonpreferring rats, Alcohol-preferring rats, Ethanol effects, Local cerebral glucose utilization",
author = "Strother, {Wendy N.} and McBride, {William J.} and Lawrence Lumeng and Li, {Ting Kai}",
year = "2005",
month = "2",
doi = "10.1016/j.alcohol.2005.03.003",
language = "English",
volume = "35",
pages = "119--128",
journal = "Alcohol",
issn = "0741-8329",
publisher = "Elsevier Inc.",
number = "2",

}

TY - JOUR

T1 - Effects of acute administration of ethanol on cerebral glucose utilization in adult alcohol-preferring and alcohol-nonpreferring rats

AU - Strother, Wendy N.

AU - McBride, William J.

AU - Lumeng, Lawrence

AU - Li, Ting Kai

PY - 2005/2

Y1 - 2005/2

N2 - Local cerebral glucose utilization (LCGU) rates, as determined by the [14C]-2-deoxyglucose (2-DG) technique, were examined after acute ethanol administration within selected brain regions of alcohol-preferring (P) and alcohol-nonpreferring (NP) rats. Adult male P and NP rats were injected with saline, 0.25 g/kg, or 1.0 g/kg ethanol, intraperitoneally (ip), 10 min before an intravenous bolus of [14C]2-DG (125 μCi/kg). Timed arterial blood samples were collected over 45 min and assayed for plasma glucose, ethanol, and [14C]2-DG levels. Image densities were determined using quantitative autoradiography and LCGU values calculated. Data were collected from several key limbic, basal ganglionic, cortical, and subcortical structures. Low-dose ethanol (0.25 g/kg) significantly decreased LCGU rates in several brain regions including the medial prefrontal cortex, olfactory tubercles, and the CA1 subregion of the hippocampus of P rats. Low-dose ethanol had no significant effects on LCGU rates in the NP rats. Moderate-dose ethanol (1.0 g/kg) also significantly lowered LCGU rates in many brain regions of P rats, including key limbic structures, such as the medial prefrontal cortex, olfactory tubercles, ventral tegmental area, basolateral nucleus of the amygdala, lateral septum, and ventral pallidum. Moderate-dose ethanol also significantly lowered LCGU rates in the medial prefrontal cortex as well as in the habenula of NP rats. All other regions were unaffected in the NP rats. These findings support the suggestion that certain central nervous system regions of P rats may be more sensitive than those of NP rats to the effects of low to intermediate doses of ethanol.

AB - Local cerebral glucose utilization (LCGU) rates, as determined by the [14C]-2-deoxyglucose (2-DG) technique, were examined after acute ethanol administration within selected brain regions of alcohol-preferring (P) and alcohol-nonpreferring (NP) rats. Adult male P and NP rats were injected with saline, 0.25 g/kg, or 1.0 g/kg ethanol, intraperitoneally (ip), 10 min before an intravenous bolus of [14C]2-DG (125 μCi/kg). Timed arterial blood samples were collected over 45 min and assayed for plasma glucose, ethanol, and [14C]2-DG levels. Image densities were determined using quantitative autoradiography and LCGU values calculated. Data were collected from several key limbic, basal ganglionic, cortical, and subcortical structures. Low-dose ethanol (0.25 g/kg) significantly decreased LCGU rates in several brain regions including the medial prefrontal cortex, olfactory tubercles, and the CA1 subregion of the hippocampus of P rats. Low-dose ethanol had no significant effects on LCGU rates in the NP rats. Moderate-dose ethanol (1.0 g/kg) also significantly lowered LCGU rates in many brain regions of P rats, including key limbic structures, such as the medial prefrontal cortex, olfactory tubercles, ventral tegmental area, basolateral nucleus of the amygdala, lateral septum, and ventral pallidum. Moderate-dose ethanol also significantly lowered LCGU rates in the medial prefrontal cortex as well as in the habenula of NP rats. All other regions were unaffected in the NP rats. These findings support the suggestion that certain central nervous system regions of P rats may be more sensitive than those of NP rats to the effects of low to intermediate doses of ethanol.

KW - Alcohol-nonpreferring rats

KW - Alcohol-preferring rats

KW - Ethanol effects

KW - Local cerebral glucose utilization

UR - http://www.scopus.com/inward/record.url?scp=20444370202&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=20444370202&partnerID=8YFLogxK

U2 - 10.1016/j.alcohol.2005.03.003

DO - 10.1016/j.alcohol.2005.03.003

M3 - Article

C2 - 15963425

AN - SCOPUS:20444370202

VL - 35

SP - 119

EP - 128

JO - Alcohol

JF - Alcohol

SN - 0741-8329

IS - 2

ER -