Effects of adenosine receptor and muscarinic cholinergic receptor agonists on cardiac protein phosphorylation. Influence of pertussis toxin

J. Neumann, P. Boknik, G. S. Bodor, Larry Jones, W. Schmitz, H. Scholz

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

In isolated 32P-labeled guinea pig ventricular cardiomyocytes phospholamban (PLB), myosin light chain 2 and the inhibitory subunit of troponin (Tnl) were identified by antibodies. In isolated 32P-labeled guinea pig ventricular cardiomyocytes isoproterenol (0.1 μmol/l) increased phosphorylation of PLB and of the inhibitory subunit of Tnl to 159 and 119% of control, respectively, without effect on the phosphorylation state of myosin light chain 2. Additionally applied carbachol or (-)-N6- phenylisopropyladenosine reduced phosphorylation of PLB and Tnl without reducing cyclic AMP content. After pretreatment of guinea pigs with pertussis toxin (18 μg/100 g b.wt.), carbachol or (-)-N6-phenylisopropyladenosine failed to reduce isoproterenol-stimulated phosphorylation of both PLB and Tnl. It is concluded that the reductions by carbachol and (-)-N6- phenylisopropyladenosine of isoproterenol-stimulated PLB phosphorylation and Tnl phosphorylation are pertussis toxin-sensitive.

Original languageEnglish (US)
Pages (from-to)1310-1318
Number of pages9
JournalJournal of Pharmacology and Experimental Therapeutics
Volume269
Issue number3
StatePublished - 1994
Externally publishedYes

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Muscarinic Agonists
Purinergic P1 Receptors
Pertussis Toxin
Cholinergic Receptors
Muscarinic Receptors
Phenylisopropyladenosine
Phosphorylation
Carbachol
Isoproterenol
Guinea Pigs
Proteins
Cardiac Myocytes
Troponin
Cyclic AMP
phospholamban
Antibodies

ASJC Scopus subject areas

  • Pharmacology

Cite this

Effects of adenosine receptor and muscarinic cholinergic receptor agonists on cardiac protein phosphorylation. Influence of pertussis toxin. / Neumann, J.; Boknik, P.; Bodor, G. S.; Jones, Larry; Schmitz, W.; Scholz, H.

In: Journal of Pharmacology and Experimental Therapeutics, Vol. 269, No. 3, 1994, p. 1310-1318.

Research output: Contribution to journalArticle

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AU - Scholz, H.

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