Effects of adenosine receptor and muscarinic cholinergic receptor agonists on cardiac protein phosphorylation. Influence of pertussis toxin

J. Neumann, P. Boknik, G. S. Bodor, L. R. Jones, W. Schmitz, H. Scholz

Research output: Contribution to journalArticlepeer-review

33 Scopus citations

Abstract

In isolated 32P-labeled guinea pig ventricular cardiomyocytes phospholamban (PLB), myosin light chain 2 and the inhibitory subunit of troponin (Tnl) were identified by antibodies. In isolated 32P-labeled guinea pig ventricular cardiomyocytes isoproterenol (0.1 μmol/l) increased phosphorylation of PLB and of the inhibitory subunit of Tnl to 159 and 119% of control, respectively, without effect on the phosphorylation state of myosin light chain 2. Additionally applied carbachol or (-)-N6- phenylisopropyladenosine reduced phosphorylation of PLB and Tnl without reducing cyclic AMP content. After pretreatment of guinea pigs with pertussis toxin (18 μg/100 g b.wt.), carbachol or (-)-N6-phenylisopropyladenosine failed to reduce isoproterenol-stimulated phosphorylation of both PLB and Tnl. It is concluded that the reductions by carbachol and (-)-N6- phenylisopropyladenosine of isoproterenol-stimulated PLB phosphorylation and Tnl phosphorylation are pertussis toxin-sensitive.

Original languageEnglish (US)
Pages (from-to)1310-1318
Number of pages9
JournalJournal of Pharmacology and Experimental Therapeutics
Volume269
Issue number3
StatePublished - Jan 1 1994
Externally publishedYes

ASJC Scopus subject areas

  • Molecular Medicine
  • Pharmacology

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